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Plant Transformation Vectors Having Street Rabies Virus (Indian Strain) Glycoprotein Gene

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Abstract

Rabies, a serious health hazard, affects central nervous system of mammals and is caused by rabies virus. The viral glycoprotein forms the external surface of virus and is the most antigenic protein. Hence the rabies glycoprotein is an ideal candidate for use in the construction of a subunit marked vaccine. With the idea to substitute the total inactivated virus with G protein in the production of vaccine, the study was undertaken to clone the full-length glycoprotein gene under the control of various constitutive and expression promoters for its expression in bacterial background and its further cloning for plant transformation.

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Abbreviations

CaMV:

Cauliflower mosaic virus

CVS:

Challange virus standard

ERA:

Evelyn, Rokitniki & Abelseth

GUS:

Glucuronidase

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Authors and Affiliations

  1. Genetic Resources and Biotechnology Division, Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow, 226015, India

    Sweta Agrawal, A. K. Shasany & S. P. S. Khanuja

Authors
  1. Sweta Agrawal
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  2. A. K. Shasany
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  3. S. P. S. Khanuja
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Correspondence to S. P. S. Khanuja.

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Agrawal, S., Shasany, A.K. & Khanuja, S.P.S. Plant Transformation Vectors Having Street Rabies Virus (Indian Strain) Glycoprotein Gene. J. Plant Biochem. Biotechnol. 14, 81–87 (2005). https://doi.org/10.1007/BF03263233

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  • DOI: https://doi.org/10.1007/BF03263233

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