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Micropropagation of Anethum graveolens L Through Axillary Shoot Proliferation

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Abstract

A protocol is described for rapid and large-scale in vitro propagation of Anethum graveolens by enhanced axillary shoot induction that was dependent on BAP supply. The synergistic combination of 0.5 mg l−1 BAP and 0.1 mg l−1 IBA induced 100% shoot formation as well as shoot number (6.6 ± 0.48 per explant). Subculturing of shoot tips of in vitro plants on multiplication medium enabled continuous production of healthy shoots with similar frequency. Rooting of shoots was achieved on a medium with 1mg l−1 IBA and 0.5 mg l−1 Kn. Micropropagated plants established in garden were uniform and identical to the donor plant with respect to morphological and cytological characteristics.

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Abbreviations

BAP:

6-benzylaminopurine

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

Kn:

Kinetin

NAA:

α-naphthalene acetic acid

MS:

Murashige and Skoog

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Author notes

  1. R. K. Sharma

    Present address: Department of Botany, SP College, Srinagar, 190 001, India

Authors and Affiliations

  1. Plant Tissue Culture Laboratory, Department of Botany, Jammu University, Jammu, 180 006, India

    R. K. Sharma, A. K. Wakhlu & M. Boleria

Authors
  1. R. K. Sharma
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  2. A. K. Wakhlu
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  3. M. Boleria
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Sharma, R.K., Wakhlu, A.K. & Boleria, M. Micropropagation of Anethum graveolens L Through Axillary Shoot Proliferation. J. Plant Biochem. Biotechnol. 13, 157–159 (2004). https://doi.org/10.1007/BF03263214

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  • DOI: https://doi.org/10.1007/BF03263214

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