Abstract
A lectin from the crude extract of seeds of Delonix regia (DRL) has been purified by ammonium sulphate fractionation followed by specific adsorption on Sephadex G-50 column and subsequent displacement with 100 mM D-glucose. The purified lectin (yield 1.41 mg g−1 dry seed) is a hetero-tetramer of 156 kD in size, consisting of four polypeptides (Mr of 32, 36, 42 and 46 kD) as detected on SDS-PAGE. It is a thermostable protein and remains active between pH 2.0–11.0. The lectin agglutinated erythrocytes of human and other primates. The hemagglutinating activity was not affected by cations and chelating agents. Of the 23 different sugars tested for specificity, maximum inhibition of the hemagglutination was shown by D-glucose. The immunological crossreactions of DRL with monospecific antibodies against SBA, Con A, PNA, DBA and PHA-E indicate that DRL is very closely related to Concanavalin A.
Abbreviations
- BMA:
-
Butea monosperma agglutinin
- DRL:
-
Delonix regia lectin
- HAU:
-
Hemagglutination unit
- PNA:
-
Peanut agglutinin
- DBA:
-
Dolichos biflorus agglutinin
- SBA:
-
Soybean agglutinin
- ConA:
-
Concanavalin A
- PHA-E:
-
Erythroagglutinin
References
Davis BJ, Ann NY Acad Sci, 121 (1964) 404.
Laemmli UK, Nature, 227 (1970) 680.
Stinissen HM, Peumans WJ & Carlier AR, Planta, 159 (1983) 105.
Osawa T, Irimura T & Kawaguchi T, Methods Enzymol, 50 (1978) 367.
Wongkham S, Wongkham C, Trisonthi C, Boonsiri P, Simasathiansophon S & Atisook K, Plant Sci, 103 (1994) 121.
Kalsi G, Das HR, Babu CR & Das RH, Biochim Biophys Acta, 1117 (1992) 114.
Suvachittanont W & Jaranchavanpet P, Planta Medical, 66 (2000) 699.
Naeem A, Khan RH, Vikram H & Akif M, Arch Biochem Biophys, 396 (2001) 99.
Martinez-Cruz M, Perez-Campos E, Zenteno E & Cordoba F, J Agric Food Chem, 51 (2003) 3783.
Bσg-Hansen TC, Anal Biochem, 56 (1973) 480.
Arsian MI & Chulavatnatol M, Bangladesh Med Res Counc Bull, 26 (2000) 23.
Hankins CN, Kindinger JI & Shannon LM, Plant Physiol, 64 (1979) 104.
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Gupta, N., Narula, A. & Srivastava, P.S. Purification and Characterization of Lectin from Seeds of Delonix regia . J. Plant Biochem. Biotechnol. 13, 141–144 (2004). https://doi.org/10.1007/BF03263210
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DOI: https://doi.org/10.1007/BF03263210