Abstract
Whitefly transmitted begomoviruses are on increase in tropical and subtropical countries. It is especially true of yellow mosaic viruses infecting legumes. Management of viral diseases requires specific detection techniques to demonstrate their presence in several other alternative and weed hosts. With this in view, nucleic acid hybridization test to detect Cowpea golden mosaic virus (CPGMV) in many crops and weed species was developed. This study showed that while probe to CPGMV DNA A detected all the begomoviruses, DNA B probe was highly specific and detected only CPGMV showing its specific nature.
References
Sharma SR & Varma A, Indian Phytopathol, 29 (1976) 421.
Varma A & Malathi VG, Ann Appl Biol, 142 (2003) 145.
Roy A & Malathi VG, Trop Agric Res, 13 (2001) 341.
Briddon RW, Mansoor S, Bedford ID, Pinner MS & Markham PG, Virus Genes, 20 (2000) 17.
Dellaporta SL, Wood VP & Hicks JB, Plant Mol Biol Rep, 1 (1983) 19.
Feinberg AP & Vogelstein, Anal Biochem, 137 (1984) 266.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Roy, A., Malathi, V.G. Development of a Specific Detection Technique for Cowpea Golden Mosaic Virus. J. Plant Biochem. Biotechnol. 13, 131–133 (2004). https://doi.org/10.1007/BF03263208
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF03263208