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Tissue Culture Propagation of Elite Plant of Aloe vera Linn

Abstract

Micropropagation protocol for an elite selection of Aloe vera syn A. barbadensis through enhanced axillary branching was standardized. Murashige and Skoog medium containing 1 mg l−1 BA and 0.2 mg l-1 IBA gave highest multiplication. Citric acid at 10mg l-1 and liquid medium improved the shoot multiplication. Hundred per cent microshoots produced rooted plantlets within 15 days of culture on hormone-free agar medium. Liquid medium during rooting stage decreased the number of shoots showing rooting response. The plants were successfully transferred in the soil and were morphologically similar to mother plants.

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Abbreviations

BA:

benzyladenine

Kn:

kinetin

IBA:

3-indole butyric acid

MS:

Murashige & Skoog

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Correspondence to K. S. Barna.

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Aggarwal, D., Barna, K.S. Tissue Culture Propagation of Elite Plant of Aloe vera Linn. J. Plant Biochem. Biotechnol. 13, 77–79 (2004). https://doi.org/10.1007/BF03263197

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Key words

  • medicinal plant
  • Aloe vera kw]micropropagation kw]axillary branching