Tissue Culture Propagation of Elite Plant of Aloe vera Linn

Short Communication

Abstract

Micropropagation protocol for an elite selection of Aloe vera syn A. barbadensis through enhanced axillary branching was standardized. Murashige and Skoog medium containing 1 mg l−1 BA and 0.2 mg l-1 IBA gave highest multiplication. Citric acid at 10mg l-1 and liquid medium improved the shoot multiplication. Hundred per cent microshoots produced rooted plantlets within 15 days of culture on hormone-free agar medium. Liquid medium during rooting stage decreased the number of shoots showing rooting response. The plants were successfully transferred in the soil and were morphologically similar to mother plants.

Key words

medicinal plant Aloe vera kw]micropropagation kw]axillary branching 

Abbreviations

BA

benzyladenine

Kn

kinetin

IBA

3-indole butyric acid

MS

Murashige & Skoog

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Copyright information

© Springer 2004

Authors and Affiliations

  1. 1.TIFAC- Centre of Relevance & Excellence (CORE) in Agro & Industrial BiotechnologyThapar Institute of Engineering & Technology (Deemed University)PatialaIndia

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