Tissue Culture Propagation of Elite Plant of Aloe vera Linn
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Micropropagation protocol for an elite selection of Aloe vera syn A. barbadensis through enhanced axillary branching was standardized. Murashige and Skoog medium containing 1 mg l−1 BA and 0.2 mg l-1 IBA gave highest multiplication. Citric acid at 10mg l-1 and liquid medium improved the shoot multiplication. Hundred per cent microshoots produced rooted plantlets within 15 days of culture on hormone-free agar medium. Liquid medium during rooting stage decreased the number of shoots showing rooting response. The plants were successfully transferred in the soil and were morphologically similar to mother plants.
Key wordsmedicinal plant Aloe vera kw]micropropagation kw]axillary branching
3-indole butyric acid
Murashige & Skoog
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