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Tagging of an Aegilops speltoides Derived Leaf Rust Resistance Gene Lr 28 with a Microsatellite Marker in Wheat

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Abstract

Leaf rust resistance gene Lr28 has been transferred form Aegilops speltoides into bread wheat on chromosome 4AL. To identify the molecular markers linked to Lr28 the available microsatellite markers for wheat chromosome arm 4AL were surveyed on near isogenic lines (NILs) of Triticum aestivum cultivars having Lr28 gene, other Lrgenes and susceptible cultivars. A null allele of Xgwm 160 marker was found to be associated with Lr28. Linkage between the marker and the Lr28 resistance gene was confirmed using F2 mapping population of cross PBW343 and HD2329 + Lr28.

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Abbreviations

NIL:

near isogenic line

SCAR:

sequence characterized amplified region

SSR:

simple sequence repeats

STMS:

sequence tagged microsatellite

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Authors and Affiliations

  1. Department of Plant Breeding, Genetics & Biotechnology, Punjab Agricultural University, Ludhiana, 141 004, India

    Yogesh Vikal, Parveen Chhuneja, Rippy Singh & H. S. Dhaliwal

Authors
  1. Yogesh Vikal
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  2. Parveen Chhuneja
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  3. Rippy Singh
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  4. H. S. Dhaliwal
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Corresponding author

Correspondence to H. S. Dhaliwal.

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Vikal, Y., Chhuneja, P., Singh, R. et al. Tagging of an Aegilops speltoides Derived Leaf Rust Resistance Gene Lr 28 with a Microsatellite Marker in Wheat. J. Plant Biochem. Biotechnol. 13, 47–49 (2004). https://doi.org/10.1007/BF03263190

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  • DOI: https://doi.org/10.1007/BF03263190

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