Abstract
Soluble proteins in six somaclones and the parent at three different stages of development of Lathyrus sativus seeds showed polypeptides of mol wts ranging from 20–165 kD on SDS-PAGE. Immature seeds showed eight distinct esterase bands and two peroxidase bands. Pattern for both the enzymes among somaclones was almost similar. During maturation a new isoform of peroxidase with high mobility appeared in all the somaclones, while one new isoform of intermediate mobility was visible in somaclones Bio L-08 and Bio I-08. Cytological analysis of all the somaclones confirmed the stability of the chromosome count of 2n=14. At metaphase I seven distinct bivalents and at anaphase I and II univalents showing complements of the seven chromosomes were visible. No structural or chromosomal aberration were observed among somaclones.
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Singh, S., Johari, R.P. & Mehta, S.L. Biochemical and Cytological Studies During Seed Development in Somaclones of Lathyrus sativus . J. Plant Biochem. Biotechnol. 6, 101–104 (1997). https://doi.org/10.1007/BF03263019
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DOI: https://doi.org/10.1007/BF03263019