Skip to main content
SpringerLink
Log in

Cloning and Characterization of a Foldback DNA Element from Antirrhinum majus

  • Published:
Journal of Plant Biochemistry and Biotechnology Aims and scope Submit manuscript

Abstract

A fold back sequence linked to a copy of the transposable element, Tam2, has been Isolated, from a genomic library constructed using partially digested and size-fractionated Antirrhinum DNA Into λ EMBL4 vector, by using Tam2 specific probe. The fold back sequence visualized by heteroduplex analysis reveals a typical stem-loop structure. The foldback element is around 1.2 kb with an average of 227 bp of Inverted termini forming the stem.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Gupta M, Bertram I, Shephard NS & Saedler H, Mol Gen Genet, 193, (1983) 373.

    Article  Google Scholar 

  2. Deumling B, Proc Natl Acad Sci, USA, 78 (1981) 338.

    Article  PubMed  CAS  Google Scholar 

  3. Kata A, Vakura K & Tanlfuji S, Nudeic Acids Res, 12 (1984) 6415.

    Article  Google Scholar 

  4. Reddy AS, Upadhyaya KC & Mukherjee SG, Indian J Biochem Biophys, 26 (1989) 131.

    PubMed  CAS  Google Scholar 

  5. Flavell R, Ann Rev Plant Physiol, 31 (1980) 569.

    Article  CAS  Google Scholar 

  6. Potter SS, Nature, 297 (1982) 201.

    Article  PubMed  CAS  Google Scholar 

  7. Brierley HL & Potter SS, Nucleic Acids Res, 13 (1985) 485.

    Article  PubMed  CAS  Google Scholar 

  8. Upadhyaya KC, Somer H, Krebbers E & Saedler H, Mol Gen Genet, 199 (1985) 201.

    Article  CAS  Google Scholar 

  9. Rigby P, Dieckermann M, Rhodes C & Berg P, J Mol Biol, 113 (1977) 237.

    Article  PubMed  CAS  Google Scholar 

  10. Benton WD & Davis RW, Science, 195 (1977) 180.

    Article  Google Scholar 

  11. Southern EM, J Mol Biol, 98 (1975) 503.

    Article  PubMed  CAS  Google Scholar 

  12. Davis RW, Simon, M & Davidson N, Methods Enzymol, 21 (1971) 413.

    Article  Google Scholar 

  13. Birnboim HC & Doly J, Nucleic Acids Res, 7 (1979) 1513.

    Article  PubMed  CAS  Google Scholar 

  14. Maniatis T, Fritsch E & Sambrook J, Molecular cloning A laboratory manual, Cold Spring Harbor Laboratory, New York (1982).

    Google Scholar 

  15. Upadhyaya KC, Indian J Genet Plant Breed, (1992) In Press.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110 067, India

    Kailash C. Upadhyaya

Authors
  1. Kailash C. Upadhyaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Upadhyaya, K.C. Cloning and Characterization of a Foldback DNA Element from Antirrhinum majus . J. Plant Biochem. Biotechnol. 1, 93–95 (1992). https://doi.org/10.1007/BF03262903

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03262903

Key words

Search

Navigation