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Detection of bcl-2/IgH Rearrangements by Quantitative-competitive PCR and Capillary Electrophoresis

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Abstract

Background: PCR is the primary method for detecting minimal residual disease in hematologic cancers. One such gene target is the bcl- 2/immunoglobulin heavy chain (IgH) translocation found in a majority of cases of follicular lymphoma.

Methods and Results: We report an accurate method for quantitative detection of the bcl- 2/IgH translocation marker of follicular lymphoma in a series of patients in various stages of remission and relapse who had been treated with a combination of ifosfamide, mitoxantrone, and etoposide (MINE) chemotherapy and monoclonal anti-CD20 antibody (Rituximab). The approach uses seminested PCR followed by analysis of the products on a fluorescent capillary electrophoresis system. The quantitation of bcl- 2/IgH translocation-positive cells was sensitive and reproducible, capable of detecting as few as five malignant cells out of 300,000 normal cells.

Conclusion: Quantitative PCR enables one to monitor the kinetics of tumor reduction in patients treated with MINE chemotherapy in combination with Rituximab.

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Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, University of California at Los Angeles School of Medicine, Los Angeles, CA, USA

    Milhan Telatar PhD & Wayne W. Grody MD,PhD

  2. Division of Hematology/Oncology, University of California at Los Angeles School of Medicine, Los Angeles, CA, USA

    Christos Emmanouilides MD, PhD

Authors
  1. Milhan Telatar PhD
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  2. Wayne W. Grody MD,PhD
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  3. Christos Emmanouilides MD, PhD
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Corresponding author

Correspondence to Wayne W. Grody MD,PhD.

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Telatar, M., Grody, W.W. & Emmanouilides, C. Detection of bcl-2/IgH Rearrangements by Quantitative-competitive PCR and Capillary Electrophoresis. Molecular Diagnosis 6, 161–168 (2001). https://doi.org/10.1007/BF03262049

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  • DOI: https://doi.org/10.1007/BF03262049

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