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Differential gene expression profiles in human THP-1 monocytes treated with Lactobacillus plantarum or Staphylococcus aureus lipoteichoic acid

  • Food Science/Microbiology
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Abstract

The lipoteichoic acid (LTA) of Staphylococcus aureus (aLTA) and Lactobacillus plantarum (pLTA) engage the same toll-like receptor 2 (TLR2) signaling pathway but exert different effects on innate immunity and inflammation. The mechanisms underlying these differential effects are not yet clear. Human oligonucleotide microarrays were used to investigate the transcriptome of human THP-1 monocytes upon exposure to aLTA or pLTA, and differential gene expression profiles were observed between the aLTA- and pLTA-treated cells. The expression level of 1,302 genes in aLTAtreated cells increased more than 2-fold; some of which have been implicated in immune or inflammatory responses, cell adhesion, cell signal transduction, transcription factors, anion transport, proteolysis, and oxidative processes. Particularly, a variety of genes that encode cytokines and chemokines, and TLR signaling-related molecules belonging to the tumor necrosis factor receptor-associated factor (TRAF), nuclear factor-kappa B, and signal transducer and activator of transcription families were remarkably up-regulated by aLTA stimulation. In contrast, pLTA treatment altered the expression of only 90 genes by more than 1.5-fold, and these genes were not correlated with innate immunity, inflammation or other related processes. The different effects mediated by aLTA and pLTA were further verified and compared by analysis of the expression of a selected group of genes, including TRAFs and some cytokines and chemokines, using real time-polymerase chain reaction and ELISA. These data suggest that aLTA and pLTA have different immunomodulatory potentials. Compared with pLTA, aLTA is a stronger stimulator and impacts the expression of many innate immunity- and/or inflammation-related genes.

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Authors and Affiliations

  1. School of Biotechnology and Institute of Life Science and Resources, Kyung Hee University, Yongin, 449-701, Republic of Korea

    Ri-Zhong Zeng, Han Geun Kim, Na Ra Kim, Min Geun Gim & Dae Kyun Chung

  2. Skin Biotechnology Center, Kyung Hee University, Yongin, 449-701, Republic of Korea

    Mi Yeon Ko, Seung Yeon Lee & Dae Kyun Chung

  3. RNA Inc., #308 College of Life Science, Kyung Hee University, Yongin, 449-701, Republic of Korea

    Chul Min Kim & Dae Kyun Chung

  4. Rubber Research Institute, Chinese Academy of Tropical Agriculture Sciences, P. R. China

    Ri-Zhong Zeng

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  1. Ri-Zhong Zeng
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  2. Han Geun Kim
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  3. Na Ra Kim
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  4. Min Geun Gim
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Correspondence to Dae Kyun Chung.

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Zeng, RZ., Kim, H.G., Kim, N.R. et al. Differential gene expression profiles in human THP-1 monocytes treated with Lactobacillus plantarum or Staphylococcus aureus lipoteichoic acid. J Korean Soc Appl Biol Chem 54, 763–770 (2011). https://doi.org/10.1007/BF03253157

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  • DOI: https://doi.org/10.1007/BF03253157

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