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Immobilization of lactate as an electroactive indicator on pencil graphite electrode for the development of a new electrochemical biosensor for the detection of lactate dehydrogenase

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Abstract

A biosensor was developed for the detection of lactate dehydrogenase (LDH) enzyme using a lactate modified pencil graphite electrode (PGE). The sensor relies on the immobilization of the lactate on PGE, and LDH detection is based on the decrease of lactate peak current following oxidation to pyruvate in the presence of LDH. Square wave voltammetric technique was used for the assay of signals in the range of −0.6 to 0.8 V and a frequency of 25 Hz for the determination of LDH. The dependence of the response was investigated in terms of reaction time, washing time and LDH and NAD+ amounts. Also, the electrochemical behavior of LDH treatment on the lactate modified PGE was studied. The electrode showed good selectivity, repeatability and an operational stability of about 90% of its original response for two weeks. Moreover, the sensor displayed a linear response range from 0.36–2.13 U μl−1 for LDH with a detection limit of 0.16 U μl−1. The response time of the LDH-treated lactate modified PGE was found to be 2 s. The relative standard deviation (RSD) obtained was 3.5% (for LDH 0.71 U μl−1 and n = 3).

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References

  1. J. Di, J. Cheng, Q. Xu, H. Zheng, J. Zhuang, Y. Sun, K. Wang, X. Mo, S. Bi, Biosens. Bioelectron. 23 (2007) 682.

    CAS  Google Scholar 

  2. A. Kaplan, L.L. Szabo, K.F. Opheim, Clinical Chemistry, 3th ed., Lea and Fediger, Philadelphia, 1988.

    Google Scholar 

  3. N. Coquelle, E. Fioravanti, M. Weik, F. Vellieux, D. Madern, J. Mol. Biol. 374 (2007) 547.

    CAS  Google Scholar 

  4. N. Santos-Alvarez, M.J. Lobo-Castanon, A.J. Miranda- Ordieres, P. Tunon-Blanco, Anal. Chim. Acta 457 (2002) 275.

    CAS  Google Scholar 

  5. C. Tarmure, R. Sandulescu, C. Ionescu, J. Pharm. Biomed. Anal. 22 (2000) 355.

    CAS  Google Scholar 

  6. M.-Y. Hong, J.-Y. Chang, H.C. Yoon, H.-S. Kim, Biosens. Bioelectron. 17 (2002) 13.

    CAS  Google Scholar 

  7. T. McCormack, G. O’Keeffe, B.D. MacCraith, R. O’Kennedy, Sensors and Actuators B 41 (1997) 89.

    Google Scholar 

  8. W. Wang, S. Han, W. Jin, J. Chromatogr. B 831 (2006) 57.

    CAS  Google Scholar 

  9. J. Bergerman, Clin. Chem. 12 (1966) 797.

    CAS  Google Scholar 

  10. M.D. Smith, C.L. Olson, Anal. Chem. 46 (1974) 1544.

    CAS  Google Scholar 

  11. W. Wang, X. Sun, W. Jin, J. Chromatogr. B 798 (2003) 175.

    CAS  Google Scholar 

  12. J. Wang, Trends Anal. Chem. 21 (2002) 226.

    CAS  Google Scholar 

  13. J. Wang, A.N. Kawde, Anal. Chim. Acta 431 (2001) 219.

    CAS  Google Scholar 

  14. J. Wang, Talanta 56 (2002) 223.

    CAS  Google Scholar 

  15. D. Demetriades, A. Economou, A. Voulgaropoulos, Anal. Chim. Acta 519 (2004) 167.

    CAS  Google Scholar 

  16. T. Kakizaki, K. Hasebe, Fresenius J. Anal. Chem. 360 (1998) 175.

    CAS  Google Scholar 

  17. A.M. Bond, P.J. Mahon, J. Schiewe, V. Vicente Beckett, Anal. Chim. Acta 345 (1997) 67.

    CAS  Google Scholar 

  18. J. Wang, A.N. Kawde, E. Sahlin, Analyst 125 (2000) 5.

    CAS  Google Scholar 

  19. J. Wang, A.N. Kawde, A. Erdem, M. Salazar, Analyst 126 (2001) 2020.

    CAS  Google Scholar 

  20. M.S. Hejazi, E. Alipour, M.H. Pournaghi-Azar, Talanta 71 (2007) 1734.

    CAS  Google Scholar 

  21. M.H. Pournaghi-Azar, E. Alipour, S. Zununi, H. Froohandeh, M.S. Hejazi, Biosen. Bioelectron. 24 (2008) 524.

    CAS  Google Scholar 

  22. R.E. Sabzi, B. Sehatnia, M.H. Pournaghi-Azar, M.S. Hejazi, J. Iran. Chem. Soc. 5 (2008) 476.

    Article  CAS  Google Scholar 

  23. H. Karadeniz, B. Gulmez, F. Sahinci, A. Erdem, G.I. Kaya, N. Unver, B. Kivcak, M. Ozsoz, J. Pharm. Biomed. Anal. 33 (2003) 295.

    CAS  Google Scholar 

  24. M. Ozsoz, A. Erdem, D. Ozkan, K. Kerman, T.J. Pinnavaia, Langmuir 19 (2003) 4728.

    CAS  Google Scholar 

  25. T.A. Bendikov, T.C. Harmon, J. Chem. Educ. 82 (2005) 439.

    CAS  Google Scholar 

  26. A. Kitajima, T. Teranishi, M. Miyake, Electrochemistry 69 (2001) 16.

    CAS  Google Scholar 

  27. P. Masawat, S. Liawruangrath, Y. Vaneesorn, B. Liawruangrath, Talanta 58 (2002) 1221.

    CAS  Google Scholar 

  28. L. Engel, W. Baumann, Fresenius J. Anal. Chem. 346 (1993) 745.

    CAS  Google Scholar 

  29. C.A. Goss, J.C. Brumfield, E.A. Irene, R.W. Murray, Anal. Chem. 65 (1993) 1378.

    CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Analytical Chemistry, Faculty of Chemistry, University of Tabriz, Iran

    M. R. Majidi & S. Gholizadeh

  2. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    M. S. Hejazi

  3. Drug Applied Research Center & Pharmaceutical Nanotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

    M. S. Hejazi

Authors
  1. M. R. Majidi
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  2. S. Gholizadeh
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  3. M. S. Hejazi
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Correspondence to M. R. Majidi or M. S. Hejazi.

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Majidi, M.R., Gholizadeh, S. & Hejazi, M.S. Immobilization of lactate as an electroactive indicator on pencil graphite electrode for the development of a new electrochemical biosensor for the detection of lactate dehydrogenase. JICS 8, 59–67 (2011). https://doi.org/10.1007/BF03246202

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