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Inhibitory effects of 1α, 25dihydroxyvitamin D3 and Ajuga iva extract on oxidative stress, toxicity and hypo-fertility in diabetic rat testes

Journal of Physiology and Biochemistry volume 64, pages 231–239 (2008)Cite this article

Abstract

The aim of the eurrent study is to investigate the therapeutic and preventive effects of 1α, 25dihydroxyvitaminD3 (1,25 (OH)2 D3) andAjuga iva (AI) extraet on diabetes toxicity in rats testes. Thus diabetic rats were treated with 1α, 25dihydroxyvitaminD3 orAjuga iva extract as both therapeutie and preventive treatments on diabetes toxicity in rats testes. Our results showed that diabetes indueed a decrease in testosterone and 17β-estradiol levels in testes and plasma. Besides, a fall in testicular antioxidant capacity appeared by a deerease in both antioxidant (superoxide dismutase (SOD), eatalase (CAT) and glutathione peroxidase (GPx) activities) and non-enzymatic antioxidant (copper (Cu), magnesium (Mg) and iron (Fe) levels). All theses changes enhanced testicular toxicity (inerease in testicular aspartate amino transaminase (AST), alanine amino transaminase (ALT), laetate dehydrogenase (LDH) activities and the lipid peroxidation and triglyeeride (TG) levels). In addition, a decrease in testicular total cholesterol (TCh) level was observed in diabetic rats testes. All the ehanges lead to a decrease in the total number and mobility of epididymal spermatozoa. The administration of 1α,25dihydroxyvitaminD3 andAjuga iva extract three weeks before and after diabetes induetion interfered and prevented diabetes toxicity in the reproduetive system. 1,25 (OH)2 D3 andAjuga iva extract blunted all changes observed in diabetic rats. To sum up, the data suggested that 1,25 (OH)2 D3 andAjuga iva extract have a protective effect on alloxan-induced damage in reproductive system by enhancing the testosterone and 17β-estradiol levels, consequenty protecting from oxidative stress, cellular toxicity and maintaining the number and motility of spermatozoids.

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References

  1. Aebi, H. (1984): Catalasein vitro.Methods enzymol,105, 121–126.

    Article  PubMed  CAS  Google Scholar 

  2. Agbaje M, Rogers DA., McVicar CM, McClure N, Atkinson AB, Mallidis C, Lewis SEM. (2007): Insulin dependent diabetes mellitus: implications for male reproductive function.Hum Reprod,22, 1871–1877.

    Article  PubMed  CAS  Google Scholar 

  3. Aybek H, Aybek Z, Rota S, Sen N, Akbulut M. (2008): The effects of diabetes mellitus, age, and vitamin E on testicular oxidative stress.Fertil and Steril,90, 755–760.

    Article  CAS  Google Scholar 

  4. Ballester J, Domínguez J, Munoz MC, Meritxell Sensat M, Rigau T, Guinovart JJ, Joan N E. Rodríguez-Gil JE. (2005): Tungstate Treatment Improves Leydig Cell Function in Streptozotocin-Diabetic Rats.J Androl,26, 706–715.

    Article  PubMed  CAS  Google Scholar 

  5. Sam J, Bhathena SJ, Manuel T Velasquez MT. (2002): Beneficial role of dietary phytoestrogens in obesity and diabetes.Am J Clin Nutr,76, 1191–1201.

    Google Scholar 

  6. Borras C, Gambini J, Gómez-Cabrera MC, Sastre J, Pallardo FV, Mann GE.,et al. (2005): 17β-Oestradiol up-regulates longevity-related, antioxidant enzyme expression via the ERK1 and ERK2 [MAPK]/NFβB cascade.Aging Cell,4, 113–118.

    Article  PubMed  CAS  Google Scholar 

  7. Bour S, Visentin V, Prévot D, Daviaud D, Saulnier-Blache JS, Guigne C, Valet P, Carpéné C. (2005): Effects of oral administration of benzylamine on glucose tolerance and lipid metabolism in rats.J Physiol Biochem,61, 371–380.

    Article  PubMed  CAS  Google Scholar 

  8. Buege JA, Aust SD. (1984): Microsomal lipid peroxidation.Methods Enzymol,105, 302–310.

    Google Scholar 

  9. Carpéné C, Bour S, Visentin V, Pellati F, Benvenuti S, Iglesias-Osma MC, García-Barrado MJ, Valet P. (2005): Amine oxidase substrates for impaired glucose tolerance correction.J Physiol Biochem,61, 405–420.

    Article  PubMed  Google Scholar 

  10. Carreau S, Bourguiba S, Delalande C, Silandre D, nSaid L, Galeraud-Denis I, Lambard S. (2008): {atEstrogen Roles in Spermatogenesis}.Immun Endoe & Metab. Agents in Med Chem, in press.

  11. Chen JX, Stinnett A. (2008): Disruption of Ang-1/Tie-2 signaling contributes to the impaired myocardial vascular maturation and angiogenesis in type II diabetic mice.Arterioscler Thromb Vasc Biol,28, 1606–1613

    Article  PubMed  CAS  Google Scholar 

  12. Duzguner V, Kaya S. (2007): Effect of zinc on the lipid peroxidation and the antioxidant defense systems of the alloxan-induced diabetic rabbits.Free Radic Biol Med,42, 1481–1486.

    Article  PubMed  CAS  Google Scholar 

  13. El-Hilaly J, Tahraoui A, Israili ZH, Lyoussi B. (2006): Hypolipidemic effects of acure and subchronic administration of an aqueous extract ofAjuga iva L. whole plant in normal and diabetic rats,J Ethnopharmacol,105, 441–448.

    Article  PubMed  Google Scholar 

  14. Hamden K, Carreau S, Ellouz F, Masmoudi H, A. Elfeki (2007): A.Protective effect of 17β-estradiol on oxidative stress and liver dysfunction in aged male rats.J Physiol Biochem,63, 195–202.

    Article  PubMed  CAS  Google Scholar 

  15. Hamden K, Carreau S, Lajmi S, Aloulou D, kchaou D, Elfeki, A. (2008): Protective effect of 17β-estradiol on hyperglycemia, stress oxidant, liver dysfunction and histological changes induced by alloxan in male rat pancreas and liver.Steroids,94, 495–501.

    Article  Google Scholar 

  16. Hamden K, Masmoudi H, Ellouz F, El Feki A, Carreau S. (2008): Protective effects of peganum harmala extracts in thiourea induced diseases in adult male rat.J Environ Biol,29, 73–77.

    PubMed  Google Scholar 

  17. Hamden K, Silandre D, Delalande C, El Feki A, Carreau S. (2008): Protective effects of estrogens and caloric restriction during aging on various rat testis parameters.Asian J Androl,10, 837–845.

    Article  PubMed  CAS  Google Scholar 

  18. Havsteen H. (2002): The biochemistry and medical significance of the flavonoids,Pharmacol Ther,96, 67–202.

    Article  PubMed  CAS  Google Scholar 

  19. Kim ST, Moley KH. (2008): Paternal effect on embryo quality in diabetic mice is related to poor sperm quality and associated with decreased glucose transporter expression.Reproduction,136, 313–322.

    Article  PubMed  CAS  Google Scholar 

  20. Koh PO. (2007): Streptozotocin-induced diabetes increases the interaction of Bad/Bcl-XL and decreases the binding of pBad/14-3-3 in rat testis,Life Sci,81, 1079–1084

    Article  PubMed  CAS  Google Scholar 

  21. Le May C, Chu K, Hu M, Ortega CS, Simpson ER, Korach KS, Tsai MJ, Mauvais-Jarvis F (2006): Estrogens protect pancreatic beta-cells from apoptosis and prevent insulin-deficient diabetes rnellitus in mice.Proc Natl Acad Sci USA,103, 9232–9237.

    Article  PubMed  Google Scholar 

  22. Lorella B, Sara M, Pietro F, Mónica M, Clara C, Gianni F, Mario M, Elisabetta B (2006) Nongenomic effects of the androgen receptor and Vitamin D agonist are involved in suppressing invasive phenotype of prostate cancer cells.Steroids,71: 304–309.

    Article  Google Scholar 

  23. Lowry OH, Rosebrough NJ, Farr AL, Randall R.J. (1955): Protein measurement with Folin phenol reagent.J Biol Chem,193, 265–275.

    Google Scholar 

  24. Maiese K. (2008): Triple play: Promoting neurovascular longevity with nicotinamide, WNT, and erythropoietin in diabetes mellitus.Biomed Pharmacother,62, 1–15.

    Article  Google Scholar 

  25. Mallidis C, Agbaje I, Rogers D, Glenn J, McCullough S, Atkinson AB, Steger K, Stitt A, McClure N. (2007): Distribution of the receptor for advanced glycation end products in the human male reproductive tract: prevalence in men with diabetes mellitus.Hum Reprod,22, 2169–2177.

    Article  PubMed  CAS  Google Scholar 

  26. Marklund, S., Marklund, G. (1975): Involvement of the superoxide anion radical in the autoxidation of pyrogallol and convenient assay for superoxide dismutase.Eur J Biochem,47, 469–474.

    Article  Google Scholar 

  27. Morimoto S, Mendoza-Rodríguez CA, Hiriart M, Larrieta ME, Vital P, Cerbón MA. (2005): Protective effect of testosterone on early apoptotic damage induced by streptozotocin in rat pancreas.J Endocrinol,187, 217–224.

    Article  PubMed  CAS  Google Scholar 

  28. Pagila DE, Valentine WN. (1967): Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase.J Lab Clin Med,70, 158–169.

    Google Scholar 

  29. Ropero A, Alonso-Magdalena P, Quesada I, Nadal A. (2008): The role of estro gen receptors in the control of energy and glucose horneostasis.Steroids,73, 874–879

    Article  PubMed  CAS  Google Scholar 

  30. Sharma A, Sharma MK, Kumar M. (2007): Protective Effect of Mentha piperita against Arsenic-Induced Toxicity in Liver of Swiss Albino Mice.Basic Clin Pharmacol Toxicol,100, 249–257.

    Article  PubMed  CAS  Google Scholar 

  31. Tai K, Need AG, MíIorowitz M, Chapman IM. (2008): Vitamin D, glucose, insulin, and insulin sensitivity.Nutrition,24, 279–285.

    Article  PubMed  CAS  Google Scholar 

  32. Tresguerres JAF, Kircev R, Tresguerres AF, Borras C, Vara E, Ariznavarreta C. (2008): Molecular mechanisms in volved in the hormonal prevention of aging in the rat.J Steroid Biochem Mol Biol,108, 318–326.

    Article  PubMed  CAS  Google Scholar 

  33. Viña J, Borras C, Gambini J, Sastre J, Pallardo FV. (2005): Why females longer than males? Importance of the upregulation of longevity-associated genes by oestrogenic compounds.FEBS,579, 2541–2545.

    Article  Google Scholar 

  34. Viña, J, Sastre J, Pallardó FV, Gambini J, Borrás C. (2008): Modulation of longevity-associated genes by estrogens or phytoestrogens.Biol Chem,389, 273–277.

    Article  PubMed  Google Scholar 

  35. Wessner M, Champion B, Gerault JP, Kaouadji N, Saidi B, Lafont R. (1992): Ecdysteroids fromAjuga iva.Phytochemistry,11, 3781–3785.

    Google Scholar 

  36. Wezeman FH, Juknelis D, Himes R, Callaci JJ. (2007). Vitamin D and ibandronate prevent cancellous bone loss associated with binge alcohol treatment in male rats,Bone,41, 639–645.

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculty of Seienees, Animal Ecophysiology, Sfax, Tunisia

    K. Hamden & A. Elfeki

  2. USC 2006 INRA- EA 2608, Biochemistry-University of Caen, France

    S. Carreau

  3. ]Biochemistry laboratory, CHU H. Bourguiba, Sfax, Tunisia

    K. Jamoussi & F. Ayadi

  4. Radio-immunology laboratory, CHU H. Bourguiba, Sfax, Tunisia

    F. Garmazi & N. Mezgenni

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  1. K. Hamden
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  2. S. Carreau
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Correspondence to K. Hamden.

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Hamden, K., Carreau, S., Jamoussi, K. et al. Inhibitory effects of 1α, 25dihydroxyvitamin D3 and Ajuga iva extract on oxidative stress, toxicity and hypo-fertility in diabetic rat testes. J Physiol Biochem 64, 231–239 (2008). https://doi.org/10.1007/BF03216108

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