Gold Bulletin

, Volume 40, Issue 3, pp 213–218 | Cite as

Protein binding to gold colloids

  • Ossi Horovitz
  • Gheorghe Tomoaia
  • Aurora Mocanu
  • Traianos Yupsanis
  • Maria Tomoaia-Cotisel
Open Access
Gold 2006 Scientific Papers

Abstract

Gold nanoparticles (size from 10 to 50 nm) were synthesized by three different methods and were characterized by UV-Vis spectroscopy and transmission electron microscopy (TEM). The shape and size distribution of gold nanoparticles were determined. The interaction of major storage protein extracted and purified from aleurone cells of barley with the gold colloids has been examined using absorption UV-Vis spectroscopy and TEM observations. These investigations evidence the binding of aleurone protein to gold nanoparticles. This binding might occur by electrostatic forces between the negatively charged citrate capped gold particles and the positively charged groups of the protein (e.g., arginine and lysine residues).

References

  1. 1.
    M. C. Daniel and D. Astruc,Chem. Rev., 2004,104, 293CrossRefGoogle Scholar
  2. 2.
    2 M. A. Hayat (Editor),Colloidal Gold: Principles, Methods, and Applications, Acad. Press, New York, 1989Google Scholar
  3. 3.
    J. Turkevitch, P. C. Stevenson and J. Hillier,Discuss. Faraday Soc., 1951,11, 55CrossRefGoogle Scholar
  4. 4.
    G. Frens,Nature: Phys. Sci., 1973,241, 20Google Scholar
  5. 5.
    J. W. Slot and H. J. Geuze,Eur. J. Cell Biol., 1985,38, 87Google Scholar
  6. 6.
    G. T. Hermanson,Bioconjugate Techniques, Acad. Press, San Diego, 1996Google Scholar
  7. 7.
    K. Yamaguchi and H. Asakawa,Anal. Biochem., 1988,172, 104CrossRefGoogle Scholar
  8. 8.
    V. Patil, R. B. Malvamkar and M. Sastry,Langmuir, 1999,15, 8197CrossRefGoogle Scholar
  9. 9.
    9 M. Brust, M. Walker, D. Bethell, D. J. Schiffrin and R. J. Whyman,J. Chem. Soc., Chem. Commun.,1994, 801Google Scholar
  10. 10.
    M. Aslam, Lei Fu, Ming Su, K. Vijayamohananb and V. P. Dravid,J. Mater. Chem., 2004,14, 1795CrossRefGoogle Scholar
  11. 11.
    G. Mie,Ann. Phys. Leipzig, 1908,25, 377CrossRefGoogle Scholar
  12. 12.
    S. Link and M. El-Sayed,J. Phys. Chem. B, 1999,103, 8410CrossRefGoogle Scholar
  13. 13.
    K. L. Kelly, E. Coronado, L. L. Zhao and G. C. Schatz,J. Phys. Chem. B, 2003,107, 668CrossRefGoogle Scholar
  14. 14.
    V. Sun and Y. Xia,Anal. Chem., 2002,74, 5297CrossRefGoogle Scholar
  15. 15.
    M. B. Mohamed, V. Volkov, S. Link and M. A. El-Sayad,Chem. Phys. Lett., 2000,317, 517CrossRefGoogle Scholar
  16. 16.
    O. Horovitz, A. Mocanu, Gh. Tomoaia, L. Olenic, Gh. Mihâilescu, O. Boroştean, A. Popoviciu, C. Crâciun, T. Yupsanis, and M. Tomoaia-Cotisel, inConvergence of Micro-Nano-Biotechnologies, Series in Micro and Nanoengineering, Romanian Acad. Press, Bucharest, 2006, pp. 133–147Google Scholar
  17. 17.
    O. Seitz, M. H. Chehimi, E. Cabet-Deliry, S. Truong, N. Felidj, C. Perruchot, S. J. Greaves and J. F. Watts,Colloids Surf. A. Physicochem.Eng.Asp., 2003,218, 225CrossRefGoogle Scholar
  18. 18.
    S. Chah, M. R. Hammond and R. N. Zare,Chemistry & Biology, 2005,12, 323CrossRefGoogle Scholar
  19. 19.
    L. Olenic, Gh. Mihâilescu, S. Pruneanu, I. Bratu, A. R. Biriş, D. Lupu, Gh. Sigartâu and L. B. Tudoran,Particulate Science and Technology, 2005,23, 79CrossRefGoogle Scholar
  20. 20.
    P. Joos, A. Tomoaia-Cotisel, A. J. Sellers and M. Tomoaia-Cotisel,Colloids and Surfaces. B. Biointerfaces, 2004,37, 83CrossRefGoogle Scholar
  21. 21.
    T. Yupsanis, S. R. Burgess, P. J. Jackson and P. R. Shewry,Journal of Experimental Botany, 1990,41, 385CrossRefGoogle Scholar
  22. 22.
    I. Bratu, M. Tomoaia-Cotisel, G. Damian and A. Mocanu,J. Optoelectronics Adv. Mat., 2007,9 (3), 672Google Scholar
  23. 23.
    M. Tomoaia-Cotisel, A. Mocanu, N. Leopold, M. Vasilescu, V. Chiş and O. Cozar,J. Optoelectronics Adv. Mat., 2007,9 (3), 637Google Scholar
  24. 24.
    S. H. Brewer, W. R. Glomm, M. C. Johnson, M. K. Knag and S. Franzen,Langmuir, 2005,21, 9303CrossRefGoogle Scholar

Copyright information

© World Gold Council 2007

Authors and Affiliations

  • Ossi Horovitz
    • 1
  • Gheorghe Tomoaia
    • 2
  • Aurora Mocanu
    • 1
  • Traianos Yupsanis
    • 3
  • Maria Tomoaia-Cotisel
    • 1
  1. 1.Faculty of Chemistry and Chemical Engineering“Babes-Bolyai” University of Cluj-NapocaCluj-NapocaRomania
  2. 2.Department of Orthopedic Surgery“Iuliu Hatieganu” University of Medicine and PharmacyCluj-NapocaRomania
  3. 3.School of Chemistry, Laboratory of BiochemistryAristotelian UniversityThessalonikiGreece

Personalised recommendations