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A novel single-nucleotide polymorphism of thevisfatin gene and its associations with performance traits in the chicken

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Abstract

Visfatin is a peptide that is predominantly expressed in visceral adipose tissue and is hypothesized to be related to obesity and insulin resistance. In this study, a novel silent single-nucleotide polymorphism (SNP) was found in exon 7 of the chickenvisfatin gene (also known asPBEF1) by single-stranded conformation polymorphism (SSCP) and DNA sequencing. In total, 836 chickens forming an F2 resource population of Gushi chicken crossed with Anka broiler were genotyped by XbaI forced RFLP, and the associations of this polymorphism with chicken growth, carcass characteristics, and meat quality were analyzed. Significant associations were found between the polymorphism and 4-week body weight (BW4), 6-week body weight (BW6), 4-week body slanting length (BSL4), fat bandwidth (FBW), breast muscle water loss rate (BWLR) and breast muscle fiber density (BFD) (P < 0.05), as well as 4-week breastbone length (BBL4) (P < 0.01). These observations suggested that the polymorphism in exon7 of thevisfatin gene had significant effects on the early growth traits of chicken.

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, 712100, Yangling, Shaanxi, P. R. China

    R. L. Han, X. Y. Lan, L. Z. Zhang, G. Ren, Y. J. Jing, M. J. Li, B. Zhang, M. Zhao, Y. K. Guo & H. Chen

  2. College of Livestock Husbandry and Veterinary Engineering, Henan Agricultural University, Henan Research Center of Breeding Resources for Poultry, 450002, Zhengzhou, P. R. China

    R. L. Han & X. T. Kang

  3. Institute of Cellular and Molecular Biology, Xuzhou Normal University, 221116, Xuzhou, P. R. China

    H. Chen

Authors
  1. R. L. Han
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  2. X. Y. Lan
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  3. L. Z. Zhang
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  4. G. Ren
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  5. Y. J. Jing
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  6. M. J. Li
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  7. B. Zhang
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  8. M. Zhao
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  9. Y. K. Guo
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  10. X. T. Kang
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  11. H. Chen
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Correspondence to H. Chen.

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Han, R.L., Lan, X.Y., Zhang, L.Z. et al. A novel single-nucleotide polymorphism of thevisfatin gene and its associations with performance traits in the chicken. J Appl Genet 51, 59–65 (2010). https://doi.org/10.1007/BF03195711

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  • DOI: https://doi.org/10.1007/BF03195711

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