Journal of Applied Genetics

, Volume 49, Issue 4, pp 321–331 | Cite as

Resistance toMelampsora larici-epitea leaf rust inSalix: analyses of quantitative trait loci

  • Ann-Christin Rönnberg-Wästljung
  • Berit Samils
  • Vasilios Tsarouhas
  • Urban Gullberg
Original Article
Received:
Accepted:

Abstract

Quantitative resistance ofSalix toMelampsora larici-epitea leaf rust was studied in 2Salix mapping populations. One population was a backcross between aS. schwerinii ×S. viminalis hybrid andS. viminalis, and the other was an F2 population betweenS. viminalis andS. dasyclados. A leaf disc bioassay was used to study the components of quantitative resistance (latent period, uredinia number, and uredinia size) to 3 isolates of the leaf rust. The analysis of quantitative trait loci (QTLs) revealed 9 genomic regions in the backcross population and 7 genomic regions in the F2 population that were important for rust resistance, with QTLs explaining 8–26% of the phenotypic variation. An important genomic region was identified for the backcross population in linkage group 2, where QTLs were identified for all resistance components for 2 of the rust isolates. Four of the QTLs had overlapping mapping intervals, demonstrating a common genetic background for latent period, uredinia diameter, and uredinia number. QTLs specific to some rust isolates and to some resistance components were also found, indicating a combination of common and specific mechanisms involved in the various resistance components. Breeding implications in relation to these findings are discussed.

Keywords

leaf disc inoculation Melampsora larici-epitea QTL mapping quantitative resistance Salix dasyclados Salix schwerinii Salix viminalis 
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Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2008

Authors and Affiliations

  • Ann-Christin Rönnberg-Wästljung
    • 1
  • Berit Samils
    • 2
  • Vasilios Tsarouhas
    • 3
  • Urban Gullberg
    • 1
  1. 1.Department of Plant Biology and Forest GeneticsSwedish University of Agricultural SciencesUppsalaSweden
  2. 2.Department of Forest Mycology and PathologySwedish University of Agricultural SciencesUppsalaSweden
  3. 3.Department of Developmental Biology, Wenner-Gren InstituteStockholm UniversityStockholmSweden

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