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Journal of Applied Genetics

, Volume 49, Issue 4, pp 321–331 | Cite as

Resistance toMelampsora larici-epitea leaf rust inSalix: analyses of quantitative trait loci

  • Ann-Christin Rönnberg-Wästljung
  • Berit Samils
  • Vasilios Tsarouhas
  • Urban Gullberg
Original Article
Received:
Accepted:

Abstract

Quantitative resistance ofSalix toMelampsora larici-epitea leaf rust was studied in 2Salix mapping populations. One population was a backcross between aS. schwerinii ×S. viminalis hybrid andS. viminalis, and the other was an F2 population betweenS. viminalis andS. dasyclados. A leaf disc bioassay was used to study the components of quantitative resistance (latent period, uredinia number, and uredinia size) to 3 isolates of the leaf rust. The analysis of quantitative trait loci (QTLs) revealed 9 genomic regions in the backcross population and 7 genomic regions in the F2 population that were important for rust resistance, with QTLs explaining 8–26% of the phenotypic variation. An important genomic region was identified for the backcross population in linkage group 2, where QTLs were identified for all resistance components for 2 of the rust isolates. Four of the QTLs had overlapping mapping intervals, demonstrating a common genetic background for latent period, uredinia diameter, and uredinia number. QTLs specific to some rust isolates and to some resistance components were also found, indicating a combination of common and specific mechanisms involved in the various resistance components. Breeding implications in relation to these findings are discussed.

Keywords

leaf disc inoculation Melampsora larici-epitea QTL mapping quantitative resistance Salix dasyclados Salix schwerinii Salix viminalis 
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References

  1. Åhman I, Larsson S, 1999. Resistensförädling iSalix för energiproduktion. Växtskyddsnotiser 2: 17–19.Google Scholar
  2. Åhman I, Bertholdsson N-O, 2001. Biomass willow cultivars and local variation in attacks by herbivores and rust. Asp Appl Biol 65: 205–214.Google Scholar
  3. Basten CJ, Weir BS, Zeng Z-B, 2001. QTL Cartographer, Version 1.15. Department of Statistics, North Carolina State University, Raleigh, NC.Google Scholar
  4. Beavis WD, 1994. The power and deceit of QTL experiments: lessons from comparative studies. Proceedings of the 49th Annual Corn And Sorghum Industry Research Conference, Chicago, IL: 250–266.Google Scholar
  5. Dowkiw A, Husson C, Frey P, Pinon J, Bastien C, 2003. Partialresistance toMelampsora larici-populina leaf rust in hybrid poplars: Genetic variability in inoculated excised leaf disk bioassay and relationship with complete resistance. Phytopathology 93: 421–427.CrossRefPubMedGoogle Scholar
  6. Dowkiw A, Bastien C, 2004. Characterization of two major genetic factors controlling quantitative resistance toMelampsora larici-populina leaf rust in hybrid poplars: strain specificity, field expression, combined effects, and relationship with a defeated qualitative resistance gene. Phytopathology 94: 1358–1367.CrossRefPubMedGoogle Scholar
  7. Dowkiw A, Bastien C, 2007. Presence of defeated qualitative resistance genes frequently has a major impact on quantitative resistance toMelampsora larici-populina leaf rust inP. interamericana hybrid poplars. Tree Genetics and Genomes 3: 261–274.CrossRefGoogle Scholar
  8. Grattapaglia D, Sederoff R, 1994. Genetic linkage maps ofEucalyptus grandis andEucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics 137: 1121–1137.PubMedGoogle Scholar
  9. Jorge V, Dowkiw A, Faivre-Rampant P, Bastien C, 2005. Genetic architecture of qualitative and quantitativeMelampsora larici-populina leaf rust resistance in hybrid poplar: genetic mapping and QTL detection. New Phytol 167: 113–127.CrossRefPubMedGoogle Scholar
  10. Larsson S, 1998. Genetic improvement of willow for the short-rotation coppice. Biomass and Bioenergy 15: 23–26.CrossRefGoogle Scholar
  11. Lascoux M, Ramstedth M, Åström B, Gullberg U 1996. Components of resistance of leaf rust (Melampsora laricii-epitea Kleb./Melampsora ribesii-viminalis Kleb.) inSalix viminalis L. Theor Appl Genet 93: 1310–1318.CrossRefGoogle Scholar
  12. Lefèvre F, Pichot C, Pinon J, 1994. Intra- and interspecific inheritance of some components of the resistance to leaf rust (Melampsora larici-epitea Kleb.) in poplars. Theor Appl Genet 88: 501–507.Google Scholar
  13. Lefèvre F, Goué-Mourier MC, Faivre-Rampant P, Villar M, 1998. A single gene cluster controls incompatibility and partial resistance to variousMelampsora larici-populina races in hybrid poplars. Phytopathology 88: 156–163.CrossRefPubMedGoogle Scholar
  14. Legionnet A, Muranty H, Lefèvre F, 1999. Genetic variation of the riparian tree speciesPopulus nigra. II. Variation in susceptibility to the foliar rustMelampsora larici-populina. Heredity 82: 318–327.CrossRefPubMedGoogle Scholar
  15. McCracken AR, Dawson M, 1994. Effect ofMelampsora rust on the growth and development ofSalix burjatica ‘Korso’ in Northern Ireland. European Journal of Forest Pathology 24: 32–39.CrossRefGoogle Scholar
  16. McCracken AR, Dawson M, 1998. Short rotation coppice willow in Northern Ireland since 1973: development of the use of mixtures in the control of foliar rust (Melampsora spp.). Eur J Forest Pathol 28: 241–250.CrossRefGoogle Scholar
  17. McCracken AR, Dawson M, 2003. Rust disease (Melampsora epitea) of willow (Salix spp.) grown as short rotation coppice (SRC) in inter- and intra-species mixtures. Ann Appl Biol 143: 381–393.CrossRefGoogle Scholar
  18. McDonald BA, Linde C, 2002. Pathogen population genetics, evolutionary potential and durable resistance. Annu Rev Phytopathol 40: 349–79.CrossRefPubMedGoogle Scholar
  19. Newcombe G, 1998. Association ofMmd1, a major gene for resistance toMelampsora medusae f. sp.deltoidae, with quantitative traits in poplar rust. Phytopathology 88: 114–121.CrossRefPubMedGoogle Scholar
  20. Pei MH, Royle DJ, Hunter T, 1996. Pathogen specialisation inMelampsora epitea var.epitea onSalix. Plant Pathol 45: 679–690.CrossRefGoogle Scholar
  21. Pei MH, Yuan ZW, Hunter T, Ruiz C, 2000. Heterogeneous nature of a ‘new’ pathotype ofMelampsora rust onSalix, revealed by AFLP. Eur J Plant Pathol 106: 771–779.CrossRefGoogle Scholar
  22. Pinon J, van Dam BC, Genetet I, de Kam M, 1987. Two pathogenic races ofMelampsora larici-populina in north-western Europe. Eur J Forest Pathol 17: 47–53.CrossRefGoogle Scholar
  23. Pinon J, Frey P, 2005. Interactions between poplar clones andMelampsora populations and their implications for breeding for durable resistance. In: Pei M, McCracken AR, eds. Rust Diseases on Willow and Poplar. Wallingford UK: CAB International: 139–154.CrossRefGoogle Scholar
  24. Rönnberg-Wästljung AC, Gunnerbeck E, 1985. Infektionsmönster och värdväxtskillnader vid Melampsora-angrepp i Salixodlingar. [Infection patterns and host differences inMelampsora attacks onSalix plantations]. Report 38. Dept. of Ecology and Environmental Sciences, Swedish University of Agricultural Sciences.Google Scholar
  25. Rönnberg-Wästljung A, Thorsén J, 1988. Inter- and intraspecific variation and genotype site interaction inSalix alba L.,S. dasyclados Wimm. andS. viminalis L. Scand J Forest Res 3: 449–463.CrossRefGoogle Scholar
  26. Rönnberg-Wästljung AC, Gullberg U, 1999. Genetics of breeding characters with possible effects on biomass production inSalix viminalis (L.). Theor Appl Genet 98: 531–540.CrossRefGoogle Scholar
  27. Rönnberg-Wästljung AC, Tsarouhas V, Semerikov V, Lagercrantz U, 2003. A genetic linkage map of a tetraploidSalix viminalis andS. dasyclados hybrid based on AFLP markers. Forest Genet 10: 185–194.Google Scholar
  28. Rönnberg-Wästljung AC, Åhman I, Glynn C, Widenfalk O, 2006. Quantitative trait loci for resistance to herbivores in willow: field experiments with varying soils and climates. Entomol Exp Appl 118: 163–174.CrossRefGoogle Scholar
  29. Samils B, Stepien V, Lagercrantz U, Lascoux M, Gullberg U, 2001. Genetic diversity in relation to sexual and asexual reproduction in populations ofMelampsora larici-epitea. Eur J Plant Pathol 107: 871–881.CrossRefGoogle Scholar
  30. Tsarouhas V, Gullberg U, Lagercrantz U, 2002. An AFLP and RFLP linkage map and quantitative trait loci (QTL) analysis of growth traits inSalix. Theor Appli Genet 105: 277–288.CrossRefGoogle Scholar
  31. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M, et al. 1995. AFLP: a new technique for DNA fingerprinting. Nucl Acids Res 23: 4407–4414.CrossRefPubMedGoogle Scholar

Copyright information

© Institute of Plant Genetics, Polish Academy of Sciences, Poznan 2008

Authors and Affiliations

  • Ann-Christin Rönnberg-Wästljung
    • 1
  • Berit Samils
    • 2
  • Vasilios Tsarouhas
    • 3
  • Urban Gullberg
    • 1
  1. 1.Department of Plant Biology and Forest GeneticsSwedish University of Agricultural SciencesUppsalaSweden
  2. 2.Department of Forest Mycology and PathologySwedish University of Agricultural SciencesUppsalaSweden
  3. 3.Department of Developmental Biology, Wenner-Gren InstituteStockholm UniversityStockholmSweden

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