Skip to main content
Log in

Identification of molecular markers for selection of supermale (YY) asparagus plants

  • Short Communication
  • Published:
Journal of Applied Genetics Aims and scope Submit manuscript

Abstract

The research was aimed to elaborate a method for selection of male plants (XY, YY) and female ones (XX) as well as for identification of supermale genotypes (YY) among male phenotypes. The population obtained by self-pollination of andromonoecious plants was analysed. In order to identify the bands differentiating the male from the female genotypes, Bulk Segregant Analysis (BSA) was carried out. Primers identified by BSA analysis were used for RAPD amplification on the template of the male and female individuals. Among the products obtained by the use of primer OPB-20, some bands were linked with sex. A band of about 700 bp was found in all female plants, and in 4 phenotypically male specimens. In the male plants, the band showed a much lower intensity, compared with the female specimens. It seems that this fragment can be linked to the X chromosome in the investigated specimens. In the female specimens with XX karyotype, template duplication occurs and hence the band intensity is twice as high as in the XY karyotype. Three male plants did not include the OPB-20-700 fragment so they could potentially have the supermale (YY) karyotype. If the obtained marker proved its usefulness for identification of supermale plants, it could become a valuable tool facilitating breeding work.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  • Biffi R, Restivo FM, Caporali A, Marziani GP, Spada A, Falavigna A, 1995. A restriction fragment length polymorphism probe for early diagnosis of gender inAsparagus officinalis L. HortScience 30: 1463–1464.

    CAS  Google Scholar 

  • Corriols L, 1984. Are asparagus all-male hybrids attractive? Asparagus Res Newsletter 2: 16–19.

    Google Scholar 

  • Corriols L, Doré C, Cassini R, 1983. Asparagus breeding: national French work. Asparagus Res Newsletter 1: 12.

    Google Scholar 

  • Doré C, 1973. Androgenėsein vitro par culture d’anthėres d’Asparagusofficinalis. Ėtat actuel des recherches. Eucarpia 4e rėunion sur la selection de l’Asperge, Versailles: 155–162.

  • Doré C, 1974. Production de plantes homozygotes males et femalles α partir d’antherės d’Asperge. C. R. Acad Sci (Paris) D 278: 2135–2138.

    Google Scholar 

  • Falavigna A, 1979. Pure lines ofA. officinalis obtained byin vitro anther culture in Italy. Proc 5th Int. Asparagus Symp., Geisenheim: 91–99.

  • Falavigna A, Casali PE, Taccomi MG, 1990. Potential ofin vitro anther culture technique for asparagus. Acta Hortic 271: 39–46.

    Google Scholar 

  • Falavigna A, Casali PE, Taccomi MG, 1996. Advances in asparagus breeding followingin vitro anther culture. Acta Hortic 415: 137–142.

    Google Scholar 

  • Jamsari A, Nitz I, Reamon-Buttner SM, Jung C, 2004. BAC-derived diagnostic markers for sex determination in asparagus. Theor Appl Genet 108: 1140–1146.

    Article  CAS  PubMed  Google Scholar 

  • Jiang Ch, Sink KC, 1997. RAPD and SCAR markers linked to the sex expression locus M in asparagus. Euphytica 94: 329–333.

    Article  CAS  Google Scholar 

  • Maestri E, Restivo FM, Marziani-Longo GP, Falavigna A, Tassi F, 1991. Isozyme gene markers in the dioecious speciesAsparagus officinalis L. Theor Appl Genet 81: 613–618.

    Article  CAS  Google Scholar 

  • Reamon-Buttner SM, Jung C, 2000. AFLP-derived STS markers for the identification of sex inAsparagus officinalis L. Theor Appl Genet 100: 432–438.

    Article  CAS  Google Scholar 

  • Reamon-Buttner SM, Schondelmaier J, Jung C, 1998. AFLP markers tightly linked to the sex locus inAsparagus officinalis L. Mol Breed 4: 91–98.

    Article  CAS  Google Scholar 

  • Sneep S, 1953. The significance of andromonoecy for the breeding ofAsparagus officinalis L. Euphytica 2: 89–95.

    Article  Google Scholar 

  • Torrey D, Peirce L, 1983. Production of haploid plantlets from anthers of asparagus. HortScience 18: 569.

    Google Scholar 

  • Williams JGK, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV, 1990. DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18: 6531–6535.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Łukasz Wolko.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Gebler, P., Wolko, Ł. & Knaflewski, M. Identification of molecular markers for selection of supermale (YY) asparagus plants. J Appl Genet 48, 129–131 (2007). https://doi.org/10.1007/BF03194670

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03194670

Keywords

Navigation