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Molecular identification of new varieties ofNierembergia linariaefolia (Graham), a native Argentinean ornamental plant

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Abstract

SixNierembergia linariaefolia clones were selected for ornamental traits during a native germplasm development program. For fingerprinting diagnosis, 13 anchored inter-simple sequence repeat (ISSR) primers and 6 amplified fragment length polymorphism (AFLP) primer-enzyme combinations were used. Both markers revealed high levels of polymorphism, enabling genetic discrimination of the accessions analyzed by using 443 informative ISSRs and 541 AFLP markers. Both molecular techniques are suitable for monitoring genetic diversity inNierembergia linariaefolia and, under our experimental conditions, they showed correlation coefficients of 0.629 for similarity matrices and of 0.649 in the cophenetic matrices. These results suggest that ISSRs are a good choice for DNA analysis inN. linariaefolia when simple manipulation and a low budget are required.

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Authors and Affiliations

  1. Instituto de Floricultura, INTA-Castelar, Argentina

    Alejandro S. Escandón, Mariana Pérez de la Torre & Silvina Soto

  2. Instituto de Recursos Biológicos, INTA-Castelar, Argentina

    Noga Zelener

Authors
  1. Alejandro S. Escandón
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  2. Noga Zelener
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  3. Mariana Pérez de la Torre
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  4. Silvina Soto
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Correspondence to Alejandro S. Escandón.

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Escandón, A.S., Zelener, N., de la Torre, M.P. et al. Molecular identification of new varieties ofNierembergia linariaefolia (Graham), a native Argentinean ornamental plant. J Appl Genet 48, 115–123 (2007). https://doi.org/10.1007/BF03194668

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  • DOI: https://doi.org/10.1007/BF03194668

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