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Effect of sulfated xylans during the interaction of [125I]-thrombin with antithrombin III or heparin cofactor II of human plasma

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An Erratum to this article was published on 01 June 1995

Summary

[125I]-Labeled thrombin was incubated with human plasma and its interactions with the two plasma protease inhibitors antithrombin III (AT-III) or heparin cofactor II (HC-II) were investigated in the presence of oat spelts xylan sulfate (OSXS), sodium pentosan polysulfate (SP-54), and the results were compared with heparin and dermatan sulfate. Addition of OSXS or SP-54 enhanced the complexation of thrombin with HC-II or with both AT-III and HC-II depending upon the concentration and the duration of the interactions of the sulfated compounds with plasma. During the 30 s interaction, OSXS and SP-54 enhanced both AT-III-thrombin and HC-II-thrombin interaction while heparin was more selective and enhanced only the AT-III-thrombin interaction. However after a 2 min interaction, heparin showed an increase in the HC-II-thrombin interaction at higher concentrations. The complexations of AT-III-thrombin and HC-II-thrombin were reversed in the presence of 200 μg/ml of SP-54 during a 30 s interaction or in presence of 100 μg/ml of OSXS during a 2 min interaction. The Western blotting method of detecting thrombin showed that during the 10 s interaction, heparin enhanced the thrombin-AT-III complex formation while OSXS enhanced the thrombin-HC-II complex formation.

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Authors and Affiliations

  1. Chemistry Department and Cooperative Agriculture Research Center, Prairie View A&M University, 77446, Prairie View, Texas, USA

    R. B. Simmons, G. R. Newton & V. M. Doctor

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  1. R. B. Simmons
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  2. G. R. Newton
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  3. V. M. Doctor
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An erratum to this article is available at http://dx.doi.org/10.1007/BF03226371.

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Simmons, R.B., Newton, G.R. & Doctor, V.M. Effect of sulfated xylans during the interaction of [125I]-thrombin with antithrombin III or heparin cofactor II of human plasma. Eur. J. Drug Metab. Pharmacokinet. 20, 73–77 (1995). https://doi.org/10.1007/BF03192291

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  • DOI: https://doi.org/10.1007/BF03192291

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