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Effect of sulfated xylans during the interaction of [125I]-thrombin with antithrombin III or heparin cofactor II of human plasma

European Journal of Drug Metabolism and Pharmacokinetics volume 20, pages 73–77 (1995)Cite this article

An Erratum to this article was published on 01 June 1995

Summary

[125I]-Labeled thrombin was incubated with human plasma and its interactions with the two plasma protease inhibitors antithrombin III (AT-III) or heparin cofactor II (HC-II) were investigated in the presence of oat spelts xylan sulfate (OSXS), sodium pentosan polysulfate (SP-54), and the results were compared with heparin and dermatan sulfate. Addition of OSXS or SP-54 enhanced the complexation of thrombin with HC-II or with both AT-III and HC-II depending upon the concentration and the duration of the interactions of the sulfated compounds with plasma. During the 30 s interaction, OSXS and SP-54 enhanced both AT-III-thrombin and HC-II-thrombin interaction while heparin was more selective and enhanced only the AT-III-thrombin interaction. However after a 2 min interaction, heparin showed an increase in the HC-II-thrombin interaction at higher concentrations. The complexations of AT-III-thrombin and HC-II-thrombin were reversed in the presence of 200 μg/ml of SP-54 during a 30 s interaction or in presence of 100 μg/ml of OSXS during a 2 min interaction. The Western blotting method of detecting thrombin showed that during the 10 s interaction, heparin enhanced the thrombin-AT-III complex formation while OSXS enhanced the thrombin-HC-II complex formation.

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Affiliations

  1. Chemistry Department and Cooperative Agriculture Research Center, Prairie View A&M University, 77446, Prairie View, Texas, USA

    R. B. Simmons, G. R. Newton & V. M. Doctor

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  1. R. B. Simmons
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  2. G. R. Newton
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  3. V. M. Doctor
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An erratum to this article is available at http://dx.doi.org/10.1007/BF03226371.

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Simmons, R.B., Newton, G.R. & Doctor, V.M. Effect of sulfated xylans during the interaction of [125I]-thrombin with antithrombin III or heparin cofactor II of human plasma. Eur. J. Drug Metab. Pharmacokinet. 20, 73–77 (1995). https://doi.org/10.1007/BF03192291

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  • DOI: https://doi.org/10.1007/BF03192291

Keywords

  • Antithrombin III
  • heparin cofactor II
  • heparin
  • SP-54
  • oat spelts xylan sulfate