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The respiration-impairing effect of rubroskyrin, a toxic metabolite

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Summary

The toxic effect of rubroskyrin, a modified bis-anthraquinone pigment fromPenicillium islandicum Sopp, on mitochondrial respiration has been studied by using isolated rat liver mitochondria, comparing with those of luteoskyrin and rugulosin which are well knownislandicum toxins. It was found that rubroskyrin exerted an uncoupling effect on mitochondrial respiration, abating the respiratory control ratio (RCR) by a dose dependent manner (UD50: 10 μM) and markedly depressed state 3 respiration at high concentrations, whereas such respiration-impairing effects of luteoskyrin and rugulosin were not detected at the concentrations tested (max. 35 μM). The involvement of redox-reaction was not detected in the uncoupling effect of the quinone pigment, rubroskyrin.

Spectroscopic study at various pHs revealed that rubroskyrin possessed a pK value within the range of physiological pH, suggesting that rubroskyrin is a weak acid-type uncoupler capable of conducting protons across mitochondrial inner membrane.

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Authors and Affiliations

  1. Department of Biochemistry, Gifu University School of Medicine, 500, Gifu, Japan

    Satoru Mori & Yoshinori Nozawa

  2. Department of Nutrition, Faculty of Wellness, Chukyo Women’s University, 474, Ohbu, Aichi, Japan

    Yukiko Sugihara, Akira Kitagawa & Kiyoshi Kawai

  3. Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Nagoya City University, 467, Nagoya

    Yukio Ogihara

Authors
  1. Satoru Mori
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  2. Yukiko Sugihara
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  3. Akira Kitagawa
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  4. Kiyoshi Kawai
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  5. Yoshinori Nozawa
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  6. Yukio Ogihara
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Mori, S., Sugihara, Y., Kitagawa, A. et al. The respiration-impairing effect of rubroskyrin, a toxic metabolite. Mycotox Res 12, 91–98 (1996). https://doi.org/10.1007/BF03192267

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  • DOI: https://doi.org/10.1007/BF03192267

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