Abstract
Naturally contaminated corn containing 450 and 54 ppb aflatoxins Bi and B2, respectively was treated with Ca(OH)2 for making tortillas. The cleaned corn and tortillas were analyzed for aflatoxins B1 and B2 by high performance liquid chromatography (HPLC) and confirmed by thin layer chromatography (TLC). The average concentrations of aflatoxins B1 and B2 in the final products (tortillas) were only 40% and 28% lower than that in starting materials (corn kernels), respectively. Aflatoxins G1 and G2 were not detected in either corn or tortilla samples.
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References
Scott PM, Kanhere SR, Tarter EJ (1985) Determination of nivalenol and deoxynivalenol (vomitoxin, DON) in cereals by gas chromatography with electron capture detection. Abstract presented at 99th Annual Meeting of the Assoc Off Anal Chem, Washington, DC, Oct 27-31, 1985.
Abbas HK, Mirocha CJ, Pawlosky RJ, Pusch DJ (1985) Effect of cleaning, milling, and baking on deoxynivalenol in wheat. Appl Environ Microbiol 50: 482–486.
Abbas HK, Mirocha CJ, Rosiles R, Carvajal M (1988) Decomposition of zearalenone and deoxynivalenol in the process of making tortillas from corn. Cereal Chem 65:15–19.
McMillian WW, Wilson DM, Mirocha CJ, Widstrom NW (1983) Mycotoxin contamination in grain sorghum from fields in Georgia and Mississippi. Cereal Chem 60:226–227.
Martinez RR (1979) Las aflatoxinas en Las tortillas. Veterinaria Mexico 10: 37–44 (In Spanish).
Price RL, Jorgensen K (1985) Effects of processing on aflatoxin levels and on mutagenic potential of tortillas made from naturally contaminated corn. J Food Sei 50:347–357.
DeVries JW, Chang HL (1982) Comparison of rapid high pressure liquid chromatographic and CB methods for determination of aflatoxins in corn and peanuts. J Assoc Off Anal Chem 65:206–209.
Association of Official Analytical Chemists. 1980. Official Methods of Analysis, 13th ed, Chapter 26. AOAC, Arlington, Virginia.
Christensen CM, Mirocha CJ, Meronuck RA (1978) Molds, Mycotoxins and Mycotoxicosis.In: Miscellaneous report 142-revised 1978. Pages 3-10. Agricultural Experiment Station, University of Minnesota, St Paul, MN 55108.
Fischbach H, Campbell AD (1965) Note on detoxification of the aflatoxins. J Assoc Of fie Agr Chem 48:28.
Ulloa-Sosa M, Schroeder HW (1969) Note on aflatoxin decomposition in the process of making tortillas from corn. Cereal Chem 46:397–400.
Scott PM (1984) Effects of food processing on mycotoxins. J Food Prot 47: 489–499.
Argumedo LM, Torreblanca A, Rosiles R, Lopez MY (1985) Determination of aflatoxins and detection of zearalenone in samples of corn from nixtamal mills in Mexico City. Tecnol Aliment 20:15–19 (In Spanish).
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Paper No 15, 913, Scientific Journal Series, Minnesota Agricultural Experiment Station, St Paul, MN 55108-
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Abbas, H.K., Mirocha, C.J., Rosiles, R. et al. Effect of tortilla-preparation process on aflatoxins B1 and B2 in corn. Mycotox Res 4, 33–36 (1988). https://doi.org/10.1007/BF03192087
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DOI: https://doi.org/10.1007/BF03192087