Abstract
A method is described for thin-layer chromatographic analysis of field samples for satratoxins G and H, theStachybotrys toxins most soluble under physiological circumstances and representing the majority ofStachybotrys atra produced toxins. Besides brine shrimp bio-assay, the conversion of these macrocyclic trichothecenes to verrucarol is used to support their identification.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Harrach B, Bata A (1982) Macrocyclic trichotheceneStachybotrys toxins isolated from field sample. Proc 5 Int IUP AC Symp Mycotox Phycotox, Verein Österreichischer Chemiker, Vienna, pp 88-91
Harrach B, Bata A, Bajmócy E, Benkö M (1983) Isolation of satratoxins from the bedding straw of a sheep flock with fatal stachybotryotoxicosis. Appl Environ Microbiol 45:1419–1422
Harrach B, Bata A, Sándor G, Ványi A (1987) Isolation of macrocyclic and non-macrocyclic trichothecenes (Stachybotrys andFusarium toxins) from the environment of 200 ill sport horses. Mycotoxin Res 3:65–68
Croft WA, Jarvis BB, Yatawara CS (1986) Airborne outbreak of trichothecene toxicosis. Atmos Environ 20:549–552
Stack ME, Eppley RM (1980) High-pressure liquid chromatographic determination of satratoxins G and H in cereal grains. J Assoc Off Anal Chem 63:1278–1281
Takitani S, Asabe Y, Kato T, Suzuki M and Ueno Y (1979) Spectrodensitometric determination of trichothecene mycotoxins with 4-(p-nitrobenzyl)pyridine on silica gel thin-layer chromatograms. J Chromatogr 172:335–342
Eppley RM (1977) Chemistry of stachybotryotoxicosis. In: Rodricks JV, Hesseltine CW, Mehlman MA (Eds) Mycotoxins in Human and Animal Health, Pathotox Publishers Inc, Park Forest South, Il, pp 285–293
Harrach B, Mirocha CJ, Pathre SV, Palyusik M (1981) Macrocyclic trichothecene toxins produced by a strain ofStachybotrys atra from Hungary. Appl Environ Microbiol 41:1428 - 1432
Bata A, Harrach B, Ujszászi K, Kis-Tamás A, Lásztity R (1985) Macrocyclic trichothecene toxins produced byStachybotrys atra strains isolated in Middle Europe. Appl Environ Microbiol 49:678 - 681
Jarvis BB, Lee YW, Cömezoglu SN, Yatawara CS (1986) Trichothecenes produced byStachybotrys atra from Eastern Europe. Appl Environ Microbiol 51: 915–918
Harrach B, Nummi M, Niku-Paavoia ML, Mirocha CJ, Palyusik M (1982) Identification of “water-soluble” toxins produced by aStachybotrys atra strain from Finland. Appl Environ Microbiol 44:494–495
Harrach B (1986) The separation and identification of the toxins ofStachybotrys isolates. Thesis. Budapest
Rodricks JV, Eppley RM (1974) Stachybotrys and stachybotryotoxicosis. In: Purchase IFH (Ed) Mycotoxins, Elsevier, New York, NY, pp 181–197
Nummi M, Niku-Paavola ML (1977) Water soluble toxins ofStachybotrys alternons. Ann Nutr Alim 31:761–770
Hajtós I, Harrach B, Szigeti G, Fodor L, Malik G, Varga J (1983) Stachybotryotoxicosis as a predisposing factor of ovine systemic pasteurellosis. Acta Vet Hung 31:181188
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Harrach, B. Method for small routine laboratories for the detection of satratoxins in straw samples. Mycotox Res 4, 20–24 (1988). https://doi.org/10.1007/BF03192085
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03192085