Abstract
Biosynthesis of trichothecenes by strains ofF. sporotriahioides KF 9 6 and KF 530 was performed on rice as a medium. Three toxins in significant amounts were produced, with yield: T-2 toxin 0.7g from 600g of drv culture, being the highest of the three metabolites, HT-2 toxin 0.06g an Neosolaniol 0.015g. Toxins were extracted with methanol from ground and defatted dry culture. Liquid/liquid partition, using chloroform/water saturated solution of sodium bicarbonate as a preliminary purification was applied. Silicagel and charcoal columns were used in further purification. Toxin were separated by preparative TLC and crystallized from methanol.
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References
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Kostecki, M., Jeleń, H., Gliński, P. et al. Biosynthesis and preparation of T-2 Toxin, HT-2 Toxin and Neosolantol. Mycotox Res 3 (Suppl 1), 65–68 (1987). https://doi.org/10.1007/BF03192031
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DOI: https://doi.org/10.1007/BF03192031