Abstract
A rapid extraction method for screening aflatoxin producing potential ofAspergillus flavus group isolates is described. The method is performed using a moist wheat medium with ca. five infected grains extracted with 2 mL of chloroform, and using thin layer chromatography. This method was proved with 95A. flavus isolates from animal feeds.
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Gimeno A (1979) Thin layer chromatographic determination of aflatoxins, ochratoxins, sterimatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin, and penitrem A. J Assoc Off Anal Chem 62:579–585.
Hara S, Fennell DI, Hesseltine CW (1974) Aflatoxin-producing strains ofAspergillus flavus detected by fluorescence of agar medium under ultraviolet light. Appl Microbiol 27:1118–1123.
Jesenská Z, Poster M, Matyásova J, Poláková O (1980) Collaborative study of the effectiveness of the screening test on APA medium for aflatoxin producing Aspergilli. Zbl Bakt Hyg I Abt Orig B 171:408–415.
Lin MT, Dianese JC (1976) A coconut-agar medium for rapid detection of aflatoxin production byAspergillus spp.. Phytopathology 66:1466–1469.
Moreno MA, Suárez G (1986) Aflatoxin-producing potential ofAspergillus flavus strains from Spanish poultry feeds. Mycopathologia 95:129–132.
Sanchis V, Viñas I, Jiménez M, Hernandez E (1983) Detectión rápida de cepas deAspergillus flavus productoras de aflatoxinas. Rev Agroquím Tecnol Aliment 23:54–59.
Torrey GS, Marth Eh (1976) Silica gel medium to detect molds that produce aflatoxins. Appl Environ Microbiol 32:376–380.
de Vogel P, Rhee R van, Koelensmid WAAB (1965) A rapid screening test for aflatoxin-synthesizing Aspergilli of theAspergillus flavus-oryzae group. J Appl Bacteriol 28:213–220.
Wicklow DT, Shotwell OL, Adams GL (1981) Use of aflatoxin-producing ability medium to distinguish aflatoxin-producing strains ofAspergillus flavus. Environ Microbiol 41:697–699.
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Moreno, M.A., del Carmen Ramos, M. & Suàrez, G. A rapid extraction method for detecting aflatoxin producing isolates. Mycotox Res 3, 33–35 (1987). https://doi.org/10.1007/BF03191983
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DOI: https://doi.org/10.1007/BF03191983