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Isolation of secondary metabolites of Aspergillus ochraceus by HPLC

Mycotoxin Research volume 2, pages 9–17 (1986)Cite this article

Abstract

A method is described for the isolation and purification of ochratoxin A, ochratoxin B, ochratoxin ß mellein, 4-hydroxymellein and penicillic acid produced byAspergillus ochraceus in a synthetic liquid medium. Ochratoxin α, which was not found in the culture medium, was obtained by acid hydrolysis of ochratoxin A. A high pressure liquid Chromatograph equipped with Lichrosorb 100 and Lichrosorb RP-18 columns and UV and/or Refractive Index detectors was used.

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References

  1. Shotwell OL, Hesseltine CW, Goulden ML (1969 Ochratoxin A. Occurrence as natural contaminant of a corn sample. Appl Microbiol 17:765–766.

    CAS  PubMed  Google Scholar 

  2. Szebioto K, Chelkowski J, Dopierala G, Godlewska B, Radomyska W (1981) Mycotoxins in cereal grain. Part I. Ochratoxin, citrinin, sterigmatocystin, penicillic acid and toxigenic fungi in cereal grain. Nahrung 25:415–421.

    Google Scholar 

  3. Mislivec PB, Dieter CT, Bruce VR (1975) Mycotoxin-producing potential of mold flora of dried beans. Appl Microbiol 29:522–526.

    CAS  PubMed  Google Scholar 

  4. Christensen CM, Fanse HA, Nelson GH, Bates F, Mirocha CJ (1967) Microflora of black and red pepper. Appl Microbiol 15:622–626.

    CAS  PubMed  Google Scholar 

  5. Yamazaki M, Maebayashi Y, Miyaki K (1970) Production of ochratoxin A by Aspergillus ochraceus isolated in Japan from moldy rice. Appl Microbiol 20: 452–454.

    CAS  PubMed  Google Scholar 

  6. Duopnik B Jr, Peckham JC (1970) Mycotoxicity of Aspergillus ochraceus to chicks. Appl Microbiol 19:594–597.

    Google Scholar 

  7. Eppley RM (1968) Screening method for zearalenone, afiatoxin and ochratoxin. J Assoc Off Anal Chem 51:74–78.

    CAS  Google Scholar 

  8. Milcszewski KE v, Engel G, Teuber M (1981) Übersicht über die wichtigen toxinbildenden Schimmelpilze und ihre Toxine. In: Reiß J (ed) Mykotoxine in Lebensmitteln, Gustav Fischer Verlag, Stuttgart, pp: 13–84.

    Google Scholar 

  9. Sansing GA, Davis ND, Diener UL (1973) Effect of time and temperature on ochratoxin A production by Aspergillus ochraceus. Can J Microbiol 19:1259–1263.

    CAS  Article  PubMed  Google Scholar 

  10. Searcy JW, Davis ND, Diener UL (1969) Biosynthesis of ochratoxin A. Appl Microbiol 18:622–627.

    CAS  PubMed  Google Scholar 

  11. Nesheim S (1969) Isolation and purification of ochratoxin A and B and preparation of their methyl and ethyl esters. J Assoc Off Anal Chem 52:975–979.

    CAS  Google Scholar 

  12. Merwe KJ van der, Steyn PS, Fourie L (1965) Mycotoxins, Part II. The constitution of ochratoxin A, B, C, metabolites of Aspergillus ochraceus Wilh. J Chem Soc: 7083–7088.

  13. Cole RJ, Moore JH, Davis ND, Kirksey JW, Diener UL (1971) 4-Hydroxymellein a new metabolite of Aspergillus ochraceus. J Agric Food Chem 19:909–911.

    CAS  Article  Google Scholar 

  14. Moore JH, Davis ND, Diener UL (1972) Mellein and 4-hydroxymellein production by Aspergillus ochraceus Wilh. Appl Microbiol 23:1067–1072.

    CAS  PubMed  Google Scholar 

  15. Ciegler A (1972) Bioproduction of ochratoxin A and penicillic acid by members of the Aspergillus ochraceus group. Can J Microbiol 18:631–636.

    CAS  Article  PubMed  Google Scholar 

  16. Engel G (1976) Untersuchungen zur Bildung von Mykotoxinen und deren quantitative Bestimmung V. Zur Synthese von Ochratoxin A und Penicillinsäure durch Aspergillus ochraceus in Abhängigkeit von Qualität und Quantität des Kohlenhydratangebotes. Milchwissenschaft 31:264–267.

    CAS  Google Scholar 

  17. Bunge I, Heller K, Röschenthaler R (1979) Isolation and purification of ochratoxin A. Z Lebensm Unters Forsch 168:457–458.

    CAS  Article  Google Scholar 

  18. Lindenfelser LA, Ciegler A (1975) Solid-substrate fermentor for Ochratoxin A production. Appl Micobiol 29:323–327.

    CAS  Google Scholar 

  19. Davis ND, Searcy JW, Diener UL (1969) Production of ochratoxin A by Aspergillus ochraceus in a semisynthetic medium. Appl Microbiol 17:742–744.

    CAS  PubMed  Google Scholar 

  20. Ferreira NP (1967) Recent advances in research on ochratoxin. Part 2. Microbiological aspects. In: Mateles R, Wogan GN (eds) Biochemistry of some food-borne microbial toxins, MIT, Press Cambridge, Mass. pp.: 157–168.

    Google Scholar 

  21. Steele JA, Davis ND, Diener UL (1973) Effect of zinc, copper, and iron on ochratoxin A production. Appl Microbiol 25:847–849.

    CAS  PubMed  Google Scholar 

  22. Cole RJ, Cox RH (1981) Handbook of toxic fungal metabolites. Academic Press, New York: 128.

    Google Scholar 

  23. Steyn PS, Holzapfel CW (1967) The synthesis of ochratoxin A and B. Tetrahedron 23:4449–4461.

    CAS  Article  PubMed  Google Scholar 

  24. Delgadillo I (1985) Kontrolle der Biosynthese von Sekundärmetaboliten bei Aspergillus ochraceus. Dissertation Mainz, FRG.

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Affiliations

  1. Departamento de Quimica, Universidade de Aveiro, 3800, Aveiro, Portugal

    Ivonne Delgadillo

Authors
  1. Ivonne Delgadillo
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Additional information

This article reports part of a study carried out at the Institut für Biochemie, Johannes-Gutenberg-Universität, Mainz, FRG, between 1980 and 1984.

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Delgadillo, I. Isolation of secondary metabolites of Aspergillus ochraceus by HPLC. Mycotox Res 2, 9–17 (1986). https://doi.org/10.1007/BF03191957

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  • DOI: https://doi.org/10.1007/BF03191957

Keywords

  • AcOH
  • Citrinin
  • Preparative HPLC
  • Mycotoxin Research
  • Penicillic Acid