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Analysis of the novel VNTR polymorphisms ofMUC8 gene

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Abstract

Analysis of mucin genes has identified the presence of several features that may represent important functional domains in mucin glycoproteins. In the central region of each mucin, there are a variable number of tandem repeats (VNTR; minisatellites). However, their genomic levels are unclear because of complex genomic properties. We report here the distribution of VNTR and polymorphic analysis ofMUC8. We searched for VNTR ofMUC8 using the Tandem Repeat Finder program and found nine VNTR motif. Six (MUC8 MS1∼MS6) among the nine VNTRs were evaluated in this study. Each VNTR inMUC8 region was analyzed in genomic DNA obtained from 200 unrelated individuals and multi-generational families. All VNTRs (MUC8 MS1, -MS2, -MS3, -MS4,-MS5 and -MS6) were genotyped as polymorphic. The degree of polymorphism within theMUC8-MS5 showed the highest heterozygosity (h = 0.786) in theMUC8 region. In order to perform a segregation analysis of the VNTRs inMUC8, we analyzed genomic DNA obtained from two generations of five families and from three generations of two families. Six of the polymorphic VNTRs were transmitted through meiosis following a Mendelian inheritance, which suggests that polymorphic VNTRs could be useful markers for paternity mapping and DNA fingerprinting.

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Authors and Affiliations

  1. Department of Biological Science, Dong-A University, 604-714, Busan, Korea

    Ji-Sun Lee, Ja-Young Kim, Eun-Kyung Ahn, Sang-Yeop Lee, Yun Hee Jeong, Se-Ra Lee & Sun-Hee Leem

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  1. Ji-Sun Lee
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  2. Ja-Young Kim
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  3. Eun-Kyung Ahn
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  4. Sang-Yeop Lee
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  5. Yun Hee Jeong
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  6. Se-Ra Lee
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  7. Sun-Hee Leem
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Correspondence to Sun-Hee Leem.

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#J-S Lee and J-Y Kim contributed equally to this work.

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Lee, JS., Kim, JY., Ahn, EK. et al. Analysis of the novel VNTR polymorphisms ofMUC8 gene. Genes & Genomics 31, 235–241 (2009). https://doi.org/10.1007/BF03191195

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  • DOI: https://doi.org/10.1007/BF03191195

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