Abstract
Photorespiration reduces carbon fixation rate, but it is an essential process in plant. Photorespiration involves reactions in chloroplasts, peroxisomes, and mitochondria. In photorepiratory peroxisome, alanine glyoxylate aminotransferase (AGT) catalyzes the conversion of alanine and glyoxylate into glycine and pyruvate, respectively. We isolated a low temperature-inducible cDNA encoding AGT from mungbean leaves. The full-length cDNA, designated asMLT92, contains an open reading frame of 1,203 nucleotides coding for a protein of 401 amino acids. Genomic DNA blotting showed that the mungbean genome has one copy ofMLT92. MLT92 mRNA was induced by low temperature and its RNA expression was not much increased by drought stress. ABA and NaCl did not induce RNA expression ofMLT92. Based on GFP/RFP targeting experiment, GFP-MLT92 fusion protein and SKL-RFP, a peroxisome marker, were colocalized to peroxisome in tobacco protoplasts. This suggests that peroxisomalMLT92 is involved in molecular response to low temperature stress.
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References
Abel S andTheologis A (1994) Transient transformation of Arabidopsis leaf protoplasts: a versatile system to study gene expression. Plant J. 5: 421–427.
Bae H, Lee DS andHwang I (2006) Dual targeting of xylanase to chloroplasts and preroxisomes as a means to increase protein accumulation in plant cells. J. Exp. Bot. 57: 161–169.
Cho C-W, Lee H-J, Chung E, Kim KM, Heo JE, Kim J-I, Chung J, Ma Y, Fukui K, Lee-D-W, Kim D-H, Chung Y-S andLee J-H (2007) Molecular characterization of the soybean L-asparaginase gene induced by low temperature stress. Mol. Cells 23: 280–286.
Chung E, Cho C-W, Kim K-Y, Kim K, Son H-A, Lee S-W, Lee Y-C, Kim K, Chung Y-S andLee J-H (2008) Enhanced tolerance against osmotic stresses ofEscherichia coli cells expressing soybean KS-type dehydrin. Gene & Genomics 30: 319–327.
Chung E, Seong E, Kim Y-C, Chung EJ, Oh S-K, Lee S, Park JM, Joung YH andChoi D (2004) A Method for High Frequency Virus-Induced Gene Silencing in Chilli Pepper (Capsicum annuum L. cv. Bukang). Mol. Cells 17: 377–380.
Church GM andGilbert W (1984) Genomic sequencing. Proc. Natl. Acad. Sci. USA 81: 1991–1995.
Corpas FJ, Barroso JB anddel Rio LA (2001) Peroxisomes as a source of reactive oxygen species and nitric oxide signal molecules in plant cells. Trends Plant Sci. 6: 145–150.
Hong KS, Lim HK, Chung EJ, Park EJ, Lee MH, Kim J-CT, Choi GJ, Cho KY andLee S-W (2007) Selection and characterization of forest soil metagenome genes encoding lipolytic enzymes. J. Microbiol. Biotechnol. 17: 1655–1660.
Liepman AH andOlsen LJ (1998) Sequence analysis of a cDNA encoding alanine: glyoxylate amino-transferase fromArabidopsis thaliana (Accession no AF063901) (PGR98113). Plant Physiol. 11: 1125.
Liepman AH andOlsen LJ (2001) Peroxisomal alanine: glyoxylate aminotransferase (AGTl) is a photorespiratory enzyme with multiple substrates inArabidopsis thaliana. Plant J. 25: 487–498.
Nakamura Y andTolbert NE (1983) Serine:glyoxylate, alanine:glyoxylate, and glutamate:glyoxylate aminotransferase reactions in peroxisomes from spinach leaves. J. Biol. Chem. 258: 7631–7638.
Rehfeld DW andTolbert NE (1972) Aminotransferases in peroxisomes from spinach leaves. J. Biol. Chem. 247: 4803–4811.
Reumann S andWeber APM (2006) Plant Peroxisomes respire in the light: Some gaps of the photorespiratory C2 cycle have become filled-Others remain. Biochim. et Biophys. Acta 1763: 1496–1510.
Saitou N andNei M (1987) The neighbor-joining method: A new method for constructing phylogenetic trees. Mol. Biol Evol. 4: 406–425.
Sambrook J andRussell DW (2000)Molecular cloning: A laboratory mannual, 3rd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Schlösser T, Gätgens C, Weber U andStahmann K-P (2004) Alanine: glyoxylate aminotransferase of Saccharomyces cerevisiae-encoding gene AGX1 and metabolic significance. Yeast 21; 63–73.
Shure M, Wessler S andFedoroff N (1983) Molecular identification and isolation of Waxy locus in maize. Cell 35: 225–233.
Somerville CR andOgren WL (1980) Photorespiration mutants of Arabidopsis thaliana deficient in serine-glyoxylate aminotransferase activity. Proc Natl. Acad. Sci. USA 77: 2684–2687.
Taler D, Galperin M, Benjamin I, Cohen Y andKenigsbuch D (2003) Plant eR genes that encode photorespiratory enzymes confer resistance against disease. Plant Cell 16: 172–184.
Taylor AO, Jepsen NM andChrsteller JT (1972) Plants under climatic stress: III. Low temperature, high light effects on photosynthetic products. Plant Physiol. 49: 798–802.
Thompson JD, Higgins DG andGibson TJ (1994) CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673–4680.
Wingler A, Lea PJ, Quick WP andLeegood RC (2000) Photorespiration: metabolic pathways and their role in stress protection, Philos. Trans. R. Soc. Long., B Biol. Sci. 355: 1517–1529.
Yoshida S (1999) A prefatory note on responses of plants to low temperature-stress. J. Plant Res. 112: 223–224
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This work was supported by a grant from the Bio-green 21 Project of RDA in Korea.
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Chung, E., Kim, K.M., Heo, J.E. et al. Molecular characterization of mungbean peroxisomal alanine glyoxylate aminotransferase gene induced by low temperature stress. Genes & Genomics 31, 11–18 (2009). https://doi.org/10.1007/BF03191133
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DOI: https://doi.org/10.1007/BF03191133