The determination of nandrolone and its metabolites in urine by gas chromatography-mass spectrometry

European Journal of Drug Metabolism and Pharmacokinetics volume 22, pages421–425(1997)Cite this article

Summary

The determination of nandrolone and its major metabolites in urine of a healthy volunteer is typically performed by fused-silica capillary column gas chromatography with electron impact quadropole mass spectrometry. Two well-known urinary metabolites of nandrolone, 19-norandrosterone and 19-norethiocholanolone, were isolated by XAD-2 adsorption from urine, eluted with methanol and separated into unconjugated and conjugated fractions. The conjugated fraction was hydrolyzed with β-glucuronidase fromEscherichia coli and the samples derivatized with MSTFA/ammonium iodide/dithioerythritol. Ion fragmentograms of thebis-trimethylsilyl derivatives of nandrolone and its metabolites displayed molecular ions of M+=420 and M+−15=405. Extraction yield and the minimum detection limit of nandrolone in urine were identified. Finally, excretion rates of nandrolone and its metabolites in urine were determined.

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Affiliations

  1. Turkish Doping Control Center, Department of Analytical Chemistry, Faculty of Pharmacy, Hacettepe University, 06100, Ankara, Turkey

    Durişehvar Özer & Aytekin Temìzer

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  1. Durişehvar Özer
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  2. Aytekin Temìzer
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Correspondence to Durişehvar Özer.

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Özer, D., Temìzer, A. The determination of nandrolone and its metabolites in urine by gas chromatography-mass spectrometry. Eur. J. Drug Metab. Pharmacokinet. 22, 421–425 (1997). https://doi.org/10.1007/BF03190980

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Keywords

  • Nandrolone
  • metabolites
  • urine
  • GC-MS