The determination of nandrolone and its metabolites in urine by gas chromatography-mass spectrometry


The determination of nandrolone and its major metabolites in urine of a healthy volunteer is typically performed by fused-silica capillary column gas chromatography with electron impact quadropole mass spectrometry. Two well-known urinary metabolites of nandrolone, 19-norandrosterone and 19-norethiocholanolone, were isolated by XAD-2 adsorption from urine, eluted with methanol and separated into unconjugated and conjugated fractions. The conjugated fraction was hydrolyzed with β-glucuronidase fromEscherichia coli and the samples derivatized with MSTFA/ammonium iodide/dithioerythritol. Ion fragmentograms of thebis-trimethylsilyl derivatives of nandrolone and its metabolites displayed molecular ions of M+=420 and M+−15=405. Extraction yield and the minimum detection limit of nandrolone in urine were identified. Finally, excretion rates of nandrolone and its metabolites in urine were determined.

This is a preview of subscription content, access via your institution.


  1. 1.

    Copera H. (1985): The history of anabolic steroids and review of clinical experience with anabolic steroids. Acta Endocrinol., 271 Suppl., 11–19.

    Google Scholar 

  2. 2.

    Di Pasquale M.G. (1984): Drug use and detection in amateur sports. MGD Press, 5–8.

  3. 3.

    Donike M. (1993): Steroid profiling in Cologne. Proceedings of 10th Cologne workshop on Doping Analysis, 7–12 March, 47–68.

  4. 4.

    Rockhold R.W. (1993): Cardiovascular toxicity of anabolic steroids. Annu. Rev. Pharmacol. Toxicol., 33, 497–520.

    Article  CAS  PubMed  Google Scholar 

  5. 5.

    Labree M. (1991): A review of anabolic steroids: uses and effects. J. Sports Med. Phys. Fitness, 31, 618–626.

    CAS  PubMed  Google Scholar 

  6. 6.

    Moss H.B., Panzak G.L., Torter R.E. (1993): Sexual functioning of male anabolic abusers. Arch. Sex Behav., 22, 1–13.

    Article  CAS  PubMed  Google Scholar 

  7. 7.

    Shackleton C.H.L., Ward R.I., Lawson A.M. (1978): Proceedings of 26th Annual Conference on Mass Spectrometry and Allied Topics, St-Louis, Missouri. 582–584.

  8. 8.

    Short R.V. (1960): Identification of 19-norandrostenedione in follicular fluid. Nature, 188, 232.

    Article  CAS  Google Scholar 

  9. 9.

    Silberzahn P., Dekenin L., Zwain I., Reffsteck A. (1985): Gas chromatography-mass spectrometry of androgens in equine ovarian follicles at ultrastructurally defined stages of development. Identification of 19-nortestosterone in follicular fluid. Endocrinology, 117, 2176.

    Article  CAS  PubMed  Google Scholar 

  10. 10.

    Reznik Y., Herrou M., Dehennin L. et al. (1987): Rising plasma levels of 19-nortestosterone throughout pregnancy. J. Clin. Endocrinol. Metab., 64, 1086–1088.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information



Corresponding author

Correspondence to Durişehvar Özer.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Özer, D., Temìzer, A. The determination of nandrolone and its metabolites in urine by gas chromatography-mass spectrometry. Eur. J. Drug Metab. Pharmacokinet. 22, 421–425 (1997).

Download citation


  • Nandrolone
  • metabolites
  • urine
  • GC-MS