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CYP4A1 gene transfection studies and the peroxisome proliferator-activated receptor: development of a high-throughput assay to detect peroxisome proliferators

European Journal of Drug Metabolism and Pharmacokinetics volume 22, pages 315–319 (1997)Cite this article

Summary

An in vitro reporter gene assay has been established to examine cytochrome P4504A1 (CYP4A1) induction. A response element from the upstream region of the rat CYP4A1 gene containing a peroxisome proliferator response element (PPRE) has been linked to the chloramphenicol acetyl-transferase (CAT) gene in a reporter vector (1). This CYP4A1 reporter construct has been co-transfected into human HepG2 cells in the presence and absence of expression vectors encoding the transcription factors PPARα and RXRα. The assay employs calcium phosphate-DNA co-precipitate mediated transfection. Reporter gene products have been quantitated using chemiluminescent based assays. We have shown that, in the presence of PPARα, the above CYP4A1 construct is transcriptionally activated by a range of structurally different peroxisome proliferators including Wy-14,643, ciprofibrate, clofibric acid and nafenopin. Our future efforts will focus on the establishment of a high-throughput assay for the detection of peroxisome proliferators. Such an assay would provide an invaluable in vitro test for the screening of developmental drug candidates prior to in vivo studies.

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Authors and Affiliations

  1. Molecular Toxicology Group, School of Biological Sciences, University of Surrey, GU2 5XH, Guildford, Surrey, UK

    S. J. Giddings & G. G. Gibson

  2. Department of Drug Metabolism, Smith Kline Beecham Pharmaceuticals, Welwyn, UK

    S. E. Clarke

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  1. S. J. Giddings
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  2. S. E. Clarke
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  3. G. G. Gibson
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Giddings, S.J., Clarke, S.E. & Gibson, G.G. CYP4A1 gene transfection studies and the peroxisome proliferator-activated receptor: development of a high-throughput assay to detect peroxisome proliferators. Eur. J. Drug Metab. Pharmacokinet. 22, 315–319 (1997). https://doi.org/10.1007/BF03190963

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