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Lipid peroxidation: the role of Ca2+ and protection by calcinine

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Summary

When calcinine (A-23187) (2 μM), a known Ca2+ ionophore, is present, a significant protection is observed to a mitochondrial suspension undergoing lipid peroxidation by Fe2+-citrate complex. A-23187 can remove Ca2+, which seems to have an important role in the lipid peroxidation process, from its ‘lesive sites’ and consequently preventing the damage. This information has importance in terms of knowing the mechanisms and avoiding the damages of lipid peroxidation that occur in some pathological cases such as tumor promotion and hemochromatosis.

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Abbreviations

EGTA:

ethyleneglycolbis (β-amino-ethyl ether) N,N’-tetraacetic acid

TPP:

tetraphenylphosphonium

HEPES:

4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid

RLM:

rat liver mitochondria; and ΔΨ transmembrane electrical potential

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Authors and Affiliations

  1. Department of Biochemistry, Institute of Chemistry, Universidade Estadual Paulista (UNESP), C.P. 355, 14801-970, Araraquara. S.P., Brazil

    R. S. Pereira

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  1. R. S. Pereira
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Pereira, R.S. Lipid peroxidation: the role of Ca2+ and protection by calcinine. European Journal of Drug Metabolism and Pharmacokinetics 21, 23–26 (1996). https://doi.org/10.1007/BF03190274

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  • DOI: https://doi.org/10.1007/BF03190274

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