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A novel isocratic HPLC method to separate and quantify acetanilide and its hydroxy aromatic derivatives: 2-, 3- and 4-hydroxyacetanilide (paracetamol or acetaminophen)

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Summary

Reverse-phase high performance liquid chromatography on a μBondapak C-18 Column has been used to separate and quantify acetanilide and its aromatic monohydroxy derivatives in the 2-, 3- and 4- positions. Separation was achieved within 22 min by using an isocratic mixture of 2-propanol: methanol: water, 8: 18: 74 (v/v). This method compares very favourably with other HPLC techniques already reported to separate acetanilide from the monohydroxy aromatic derivatives.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Medicine, University of Chile, P.O. Box 70086, 7, Santiago, Chile

    J. Mancilla, E. Valdes & L. Gil

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  1. J. Mancilla
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  2. E. Valdes
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  3. L. Gil
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Mancilla, J., Valdes, E. & Gil, L. A novel isocratic HPLC method to separate and quantify acetanilide and its hydroxy aromatic derivatives: 2-, 3- and 4-hydroxyacetanilide (paracetamol or acetaminophen). European Journal of Drug Metabolism and Pharmacokinetics 14, 241–244 (1989). https://doi.org/10.1007/BF03190105

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  • DOI: https://doi.org/10.1007/BF03190105

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