Summary
Cellular distribution of the lipophilic environmental pollutant, 2,2′, 4,4′, 5,5 ′-hexachlorobiphenyl (6-CB), was determined by autoradiography afterin vitro uptake of thel4C-labeled compound into liver and skin tissue preparations. Light microscopic data of cryostat sections showed that 6-CB is homogeneously distributed in liver tissue. In skin the distribution pattern depended on the conditions of incubation. If skin slices were incubated with 6-CB prior to preparation of cryostat thin sections, the epidermis was practically free of 6-CB and the radioactivity was found mainly in the stratum reticulare of the dermis. If, however, cryostat sections of skin were directly incubated, 6-CB was more homogeneously distributed with an accumulation in the epidermis. Liver and skin sections delipidated with acetone or 95% ethanol prior to incubation with 6-CB took up very little of the compound. Delipidation of sections preincubated with 6-CB resulted in total extraction of 6-CB. The results suggest that human stratum corneum is a barrier to the penetration of 6-CB and that this compound is located in lipid structures of liver and skin.
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References
Matthews H.B. and Anderson M.W. (1975): Effects of Chlorination on the Distribution and Excretion of Polychlorinated Biphenyls, Drug Metab. Dispos.,3, 371–380.
Mühlebach S. and Bickel M.H. (1981): Pharmacokinetics in rats of 2,4,5,2′,4′,5′-hexachlorobiphenyl, an unmetabolizable lipophilic compound, Xenobiotica,11, 249–257.
Mühlebach S., Wyss P.A. and Bickel M.H. (1985): Comparative adipose tissue kinetics of thiopental, DDE and 2,4,5,2′,4′,5′-hexachlorobiphenyl in the rat, Xenobiotica,15, 485–491.
Wyss P.A., Mühlebach S. and Bickel M.H. (1982): Pharmacokinetics of 2,2′,4,4′,5,5′-Hexachlorobiphenyl in Rats with Decreasing Adipose Tissue Mass, Drug Metab. Dispos.,10, 657–661.
Di Francesco C. and Bickel M.H. (1985): Uptake in vitro of Lipophilic Model Compounds into Adipose Tissue Preparations and Lipids, Biochem. Pharmacol.,34, 3683–3688.
Rodbell M. (1964): Metabolism of Isolated Fat Cells, J. Biol. Chem.,239, 375–380.
Ashworth C.T., Leonard J.S., Eigenbrodt E.H. and Wrightsman F.J. (1966): Hepatic intracellular osmiophilic droplets. Effect of lipid solvents during tissue preparation, J. Cell. Biol.,31, 301–318.
Stein O. and Stein J. (1967): Lipid synthesis, intracellular transport, storage and secretion. I. Electronmicroscopic radioautographic study of liver after injection of tritiated palmitate or glycerol in fasted and ethanol-treated rats, J. Cell. Biol.,33, 319–339.
Luft J.H. (1961): Improvement in epoxy resin embedding methods, J. Biophys. Biochem. Cytol.,9, 409–414.
Vickers-Putnam A.E.M. (1983): The Disposition and Biotransformation of Polychlorinated biphenyl Congeners in Isolated Rat Hepatocytes, Thesis, University of Arizona.
Fleischer S. and Fleischer B. (1967): Removal and Binding of Polar Lipids in Mitochondria and other Membrane Systems, Methods Enzymol.,10, 406–431.
Polak J.M. and Van Noorden S., Eds. (1983): Immunocytochemistry, John Wright & Sons, Bristol (UK).
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di Francesco, C., Gerber, H.A. & Bickel, M.H. Autoradiographic study of the localization of 2,2′, 4,4′, 5,5′-hexachlorobiphenyl in liver and skin tissue after in vitro uptake. Eur. J. Drug Metab. Pharmacokinet. 13, 241–245 (1988). https://doi.org/10.1007/BF03190085
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DOI: https://doi.org/10.1007/BF03190085