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Influence of partial hepatectomy in rats on the activity of hepatic microsomal enzymatic systems

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Summary

The influence of partial hepatectomy on the activity of the hepatic microsomal enzymatic systems was determined in rats. Cytochrome P-450, cytochrome b5, four mixed functional oxidase (MFO) activities (microsomal aniline hydroxylase,p-nitroanisole O-demethylase, aminopyrine N-demethylase and NADPH cytochrome c reductase) and glutathione levels were measured in unhepatectomized rats (control group) and in hepatectomized rats 12 h, 24 h, 3 days and 6 days after 70% hepatectomy. Following surgery the remaining lobes of the liver grow rapidly in order to restore the original liver mass. Partial hepatectomy significantly reduces cytochrome P-450 and b5 content in the remaining liver as well as the four MFO activities studied. But when the enzymatic systems are expressed as nmoles/mg microsomal protein, only cytochrome P-450 shows statistical differences. The hepatic biotransformation capacity of drugs and xenobiotics decreases during the regeneration period due to the reduction of hepatic mass rather than because of a reduction of their metabolic capacity. Glutathione levels are increased after partial hepatectomy but increased glutathione-dependent protector mechanisms are not expected.

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Authors and Affiliations

  1. Laboratory of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of the Basque Country, C/ Marqués de Urquijo s/n, 01006, Vitoria, Spain

    A. M. Maza, A. R. Gascon, M. B. Calvo, R. M. Hernandez & J. L. Pedraz

  2. Department of Physiology, Faculty of Pharmacy, University of Salamanca, Spain

    M. A. Monte & J. J. G. Marin

  3. Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, University of Salamanca, Spain

    A. Dominguez-Gil

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  1. A. M. Maza
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Maza, A.M., Gascon, A.R., Calvo, M.B. et al. Influence of partial hepatectomy in rats on the activity of hepatic microsomal enzymatic systems. European Journal of Drug Metabolism and Pharmacokinetics 22, 15–23 (1997). https://doi.org/10.1007/BF03189780

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  • DOI: https://doi.org/10.1007/BF03189780

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