Summary
Sputum collected from patients with respiratory diseases were examined for presence of benzo(a)pyrene hydroxylase (BPH) activity. The human bronchial mucus used in these studies had significant capability to metabolize benzo(a)pyrene. Clarification of the sputum by agents such as N-acetylcysteine or pancreatin in presence of antibiotics was found to be essential for the detection of BPH activity.In vitro incubation of the clarified human bronchial mucus with benzoflavone caused inhibition, while 7,8-dimethylbenzanthracene induced BPH enzyme activity.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Pelkonen O. (1976): Metabolism of benzo(a)pyrene in human adult and fetal tissues. In Fruedenthal R. and Jones P.W., eds. Carcinogenesis, A Comprehensive Survey, Vol. 1, pp. 9–21.
Kuntzman R., Mark L.C., Brand L., Jacobson M., Levin W. and Conney A.H. (1966): Metabolism of drugs and carcinogens by human liver enzymes. J. Pharmacol. Exp. Therap.,152, 152–156.
Nebert D.W. and Gelboin H.V. (1969): Thein vivo andin vitro induction of arylhydrocarbon hydroxylase in mammalian cells of different species, tissues, strains and developmental and hormonal status. Arch. Biochem. Biophys.,134, 76–89.
Booth J., Keysall G.R., Kalyani P.L. and Sims P. (1974): The metabolism of polycyclic hydrocarbons by cultured lymphocytes. Febs Lett.,43, 341–344.
Bast R.C., Okunda T., Flotkin E. Tarone R., Rapp J.H. and Gelboin H.V. (1976): Development of an assay for arylhydrocarbon (benzo(a)pyrene) hydroxylase in human peripheral blood monocytes. Cancer Res.,36, 167–174.
Cantrell E.T., Warr G.A., Busbee D.L. and Martin R.R. (1973): Induction of arylhydrocarbon hydroxylase activity in human pulmonary alveolar macrophages by cigarette smoking. J. Clin. Invest.,52, 1881–1884.
Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. (1951): Protein measurement with folin phenol reagent. J. Biol. Chem.,193, 265–275.
Depierre J.W., Moron M.S., Johannesen and Emster L. (1975): A reliable sensitive and convenient radioactive assay for benzo(a)pyrene monooxygenase. Anal. Biochem.,63, 470–484.
Roberts G.P. (1976): The role of disulphide bonds in maintaining the gel structure of bronchial mucus. Arch. Biochem. Biophys.,173, 528–537.
Roberts G.P. (1978): Chemical aspects of respiratory mucus. Br. Med. Bull.,34, 39–41.
Nebert D.W. and Gelboin H.V. (1968): Substrate-inducible microsomal aryl-hydroxylase in mammalian cell culture. J. Biol. Chem.,243, 6243–6249.
Nebert D.W., Robinson J.R., Niwa A., Kumaki and Poland A. (1975): Genetic expression of arylhydrocarbon hydroxylase activity in the mouse. J. Cell Physiol.,85, 393–414.
Whitlock Jr. J.P., Cooper H.L. and Gelboin H.V. (1972): Arylhydrocarbon (benzo pyrene) hydroxylase is stimulated in human lymphocytes by mitogens and by benzanthracene. Science,177, 618–619.
Kellerman G., Luvten-Kellerman M. and Shaw C.R. (1973): Genetic variation of arylhydrocarbon hydroxylase in human lymphocytes. Am. J. Human Genetics,25, 327–331.
Kellerman G., Shaw C.R. and Luyten-Kellerman M. (1973): Arylhydrocarbon hydroxylase induicibility and bronchogenic carcinoma. New Engl. J. Med.,289, 934–937.
Bresnick E. (1939): Drug Metabolism: Speculation on the future. Drug Metab. Revs.,10, 311–313.
Gupta R.K. (1981): Value of sputum cytology in the differential diagnosis of alveolar cell carcinoma from bronchogenic adenocarcinoma. Acta Cytologica,25, 255–258.
Nebert D.W. and Gelboin H.V. (1968): Substrate-inducible microsomal arylhydroxylase in mammalian cell culture. I. Assay and properties of induced enzyme. J. Biol. Chem.,243, 6242–6249.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kapoor, A., Batra, J.K., Luthra, U.K. et al. Benzo(a)pyrene hydroxylase activity in human bronchial mucus. European Journal of Drug Metabolism and Pharmacokinetics 11, 33–37 (1986). https://doi.org/10.1007/BF03189773
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF03189773