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Acetylation of procainamide and isoniazio by a rat liver-N-acetyl-transferase

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Summary

The genetic variation of the enzyme isoniazid-N-acetyltransferase has been studied in man and other species. Homogenates of liver samples of adult LEW rats also acetylate procainamide. The kinetic properties of isoniazid and procainamide acetylation are similar and are consistent with a ping-pong Bi-Bi mechanism. The experimental data support the hypothesis that one acetyltransferase catalyses both procainamide and isoniazid. Furthermore, a simple spectrophotometric assay to determine procainamide acetylation is presented.

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Authors and Affiliations

  1. Institute of Human Genetics, Hufelandstr, Essen 1, Germany

    Evelyn Kattner

  2. Centre of Human Genetics and Genetic Counselling, University of Bremen, NW 2, D 2800, Bremen 33

    Rolf Dubbels & Werner Schloot

Authors
  1. Evelyn Kattner
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  2. Rolf Dubbels
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  3. Werner Schloot
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Kattner, E., Dubbels, R. & Schloot, W. Acetylation of procainamide and isoniazio by a rat liver-N-acetyl-transferase. European Journal of Drug Metabolism and Pharmacokinetics 6, 81–84 (1981). https://doi.org/10.1007/BF03189472

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  • DOI: https://doi.org/10.1007/BF03189472

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