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Use of electrophoresis and immunoelectrophoresis in taxonomic and pollution studies

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Proceedings: Animal Sciences

Abstract

Studies were conducted on the electrophoresis of blood serum and eye lens proteins of 5 fishes and immunoelectrophoresis of the soluble lens proteins of 10 fishes. The effects of a toxic pollutant (mercury) on the electrophoretic patterns of the serum, haemoglobin and eye lens proteins of a euryhaline fishTilapia mossambica (Peters) has also been studied. The use of fish blood morphology as a biological index of water quality has been indicated.

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References

  • Anifinsen C B 1961 The influences of three dimensional configuration on the chemical reactivity and stability of proteins;J. Polym. Sci. 49 31–49

    Article  Google Scholar 

  • Allison A C 1959 Metabolic polymorphisms in mammals and their bearing on problems of biochemical genetics;Am. Nat. 93 5–16

    Article  CAS  Google Scholar 

  • Badawi H K 1971 Electrophoretic studies of serum proteins of four tilapia species (Pisces);Mar. Biol. 8 96–98

    Article  CAS  Google Scholar 

  • Barrett I and Williams A A 1967 Soluble lens proteins of some scombroid fishes;Copeia 1967 468–471

    Article  Google Scholar 

  • Bouck G R 1966Changes in blood and muscle composition of rock bass (Ambloplites rupestris) as physiological criteria of stressful conditions, Ph.D. Thesis, Michigan State University, East Lansing, Michigan. 155 p

    Google Scholar 

  • Bouck G R and Ball R C 1967 Distribution of low mobility proteins in the blood of fishes;J. Fish. Res. Bd. Canada 24 695–697

    CAS  Google Scholar 

  • Brett J R 1958 Implications and assessments of environmental stress, inThe investigations of fish power problems, H R MacMillan lectures in fisheries, (ed) P A Larkin, (Vancouver: British Columbia Press) pp. 69–83

    Google Scholar 

  • Deligny W 1969 Serological and biochemical studies on fish populations;Oceanogr. Mar. Biol. Annu. Rev. 7 411–513

    CAS  Google Scholar 

  • Deutsch H F and Mcshan W H 1949 Biophysical studies of blood plasma proteins XII. Electrophoretic studies of the blood serum proteins of some lower animals;J. Biol. Chem. 180 219–234

    PubMed  CAS  Google Scholar 

  • Dobzhansky T 1962Mankind evolving (New Haven, Connecticut: Yale University Press) pp. 381

    Google Scholar 

  • Eckroat L R 1971 Lens protein polymorphisms in Hatchery and Natural populations of brook troutSalvelinus frontinalis (Mitchell);Trans. Am. Fish. Soc. 100 527–536

    Article  CAS  Google Scholar 

  • Eckroat L R and Wright J E 1969 Genetic analysis of soluble lens protein polymorphism in brook troutSalvelinus fontinalis;Copeia 3 466–473

    Article  Google Scholar 

  • Fujino K 1970 Immunological and biochemical genetics of tunas;Trans. Am. Fish. Soc. 99 152–178

    Article  CAS  Google Scholar 

  • Greiffendorf G and Bech S L 1969 A genetic investigation of eye lens protein variants among inbred mouse strains;Am. Zool. 9 614

    Google Scholar 

  • Griffith R W, Umminger B L, Grant B F, Pang K T and Pickford G E 1974 Serum composition of the coelacanthLatimeria chalumnae Smith;J. Exp. Zool. 187 87–102

    Article  PubMed  CAS  Google Scholar 

  • Hatting J 1974 The plasma proteins ofLabeo umbratus (Smith) andLabeo capensis (Smith);J. Fish. Biol. 6 439–446

    Article  Google Scholar 

  • Hektoen L 1922 The specific precipitin reaction of the normal and cataractous lens;J. Infect. Dis. 31 72–78

    Google Scholar 

  • Krauel K K and Ridgway G J 1963 Immunoelectrophoretic studies of red salmon (Oncorhynchus nerka) serum;Int. Arch. Allergy. Appl. Immun. 23 246

    Article  CAS  Google Scholar 

  • Landsteiner K 1901 Uber agglinationsercheinungen normalen menschlichen Blutes;Wien. Klin. Wochenschr. 14 1132–1134

    Google Scholar 

  • Love R M 1970The chemical biology of fishes (London: Academic Press) 547 p.

    Google Scholar 

  • Lumry R and Eyring H 1954 Conformation changes of proteins;J. Phys. Chem. 58 110

    Article  CAS  Google Scholar 

  • Lysak A and Wojcik K 1960 Electrophoretic investigations on the blood of carp fed with food containing various protein amounts;Acta Hydrobiol. vol II

  • Markert C L and Moller F 1959 Multiple forms of enzymes: Tissue, ontogenetic and species specific patterns;Proc. Natl. Acad. Sci. U.S.A. 45 753

    Article  PubMed  CAS  Google Scholar 

  • Manski W, Auerbach T P and Halbert S P 1960 On the phylogenetic pathways of lens proteins;Fed. Proc. Fed. Am. Soc. Exp. Biol. 19 334

    Google Scholar 

  • Manski W, Halbert S P and Auerbach T P 1961 Immunochemical analysis of lens protein separations;Arch. Biochem. Biophys. 92 512

    Article  PubMed  CAS  Google Scholar 

  • Manski W, Halbert S P and Auerbach T P 1964 Immunochemical analysis of the phylogeny of lens proteins, inTaxonomic biochemistry and serology (ed.) C A Leone (New York: The Ronald Press)

    Google Scholar 

  • Menezes M R 1979Morphometry and serology of some fishes from the Goa region. M.Sc. thesis, University of Bombay, 144 p

  • Menezes M R 1980a Soluble lens protein polymorphism in oil sardineSardinella longiceps Val;Mahasagar-Bull Natn. Inst. Oceanogr. 13 183–185

    CAS  Google Scholar 

  • Menezes M R 1980b Immunochemical analysis of soluble lens proteins in some Marine fishes;Indian J. Mar. Sci. 9 63–65

    CAS  Google Scholar 

  • Menezes M R 1981Studies on some physiological changes in fishes from polluted and unpolluted environments, Ph.D. Thesis, University of Bombay 204 p

  • Menezes M R and Qasim S Z 1983a Determination of acute toxicity levels of mercury to the fishTilapia mossambica (Peters);Proc. Indian Acad. Sci. (Anim. Sci.)92 375–380

    Article  CAS  Google Scholar 

  • Menezes M R and Qasim S Z 1983b Growth efficiencies ofTilapia mossambica (Peters) in relation to the toxicant (mercury);Mahasagar (in press)

  • Menezes M R and Qasim S Z 1984 Effects of mercury accumulation on the electrophoretic patterns of the serum, haemoglobin and eye lens proteins ofTilapia mossambica (Peters);Water Res. 18 153–161

    Article  CAS  Google Scholar 

  • Nuttall G H F 1901 The new biological test for blood in relation to zoological classification;Proc. R. Soc. (London)B69 150–153

    Article  Google Scholar 

  • Nuttall G H F 1904Blood immunity and blood relationships (Cambridge: University Press)

    Google Scholar 

  • Pirie A and R Van Heyningen 1956Biochemistry of the eye; (Springfield: Charles C Thomas) 111 p

    Google Scholar 

  • Post G 1963 The immune response of rainbow trout (Salmo gairdneri) toAeromonas hydrophilus);Utah State Dep. Fish. Game Inform. Bull. 63 1–82

    Google Scholar 

  • Post G 1966 Serum proteins and antibody production in rainbow trout (Salmo gairdneri);J. Fish. Res. Bd. Canada 23 1957–1963

    CAS  Google Scholar 

  • Ridgway G J, Klontz G W and Matsumoto G 1962 Intraspecific differences in serum antigens of red salmon demonstrated by immunochemical methods;Bull. No. 8,Int. North Pacific Fisheries Commission

  • Saito K 1957 Biochemical studies on the fish blood. On the seasonal variations of serum protein components of cultured fish;Bull. Jpn. Soc. Scient. Fish. 22 768–777

    CAS  Google Scholar 

  • Sanders B G 1964 Electrophoretic studies of serum proteins of three trout species and the resulting hybrids within the family salmonidae, inTaxonomic biochemistry and serology (ed.) C A Leone (New York: The Ronald Press)

    Google Scholar 

  • Selye H 1950Stress (Montreal Quebec: Acta Inc.) 822 p

  • Shaw C R 1965 Electrophoretic variation in enzymes;Science 149 936

    Article  PubMed  CAS  Google Scholar 

  • Sindermann C J and Mairs D F 1958 Serum protein changes in diseased sea herring;Anat. Rec. 131 599–600

    Google Scholar 

  • Smith A C 1962 The electrophoretic characteristics of albacore, blue fin tuna and kelpbass eye lens proteins;Calif. Fish. Game Bull. 48 199–201

    CAS  Google Scholar 

  • Smith A C 1966 Electrophoretic studies of eye lens proteins from marine fishes Ph.D. Thesis, University of California, Irvine,Diss. Abstr. 28 1968 98 p

  • Smith A C and Goldstein R A 1967 Variation in protein composition of the eye lens nucleus in ocean whitefish,Caulolatilus princeps;Comp. Biochem. Physiol. 23 533–539

    Article  PubMed  CAS  Google Scholar 

  • Smith A C 1971 The soluble proteins in eye lens nuclei of albacore, blue fin tuna and bonito;Comp. Biochem. Physiol. 39 719–724

    Article  CAS  Google Scholar 

  • Summerfelt R C 1966 Homology of serum proteins of golden shiner (Notemigonus crysoleucas) and man;Trans. Am. Fish. Soc. 95 272–279

    Article  CAS  Google Scholar 

  • Thomas M L H and McCrimman H R 1964 Variability in paper electrophoretic patterns of the serum of landlocked sea lamprey,Petromyzon marinus;J. Fish. Res. Bd. Can. 21 239–246

    CAS  Google Scholar 

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Authors and Affiliations

  1. National Institute of Oceanography, Dona Paula, 403 004, Goa, India

    Maria R Menezes

  2. Department of Ocean Development, South Block, 110011, New Delhi, India

    S Z Qasim

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  1. Maria R Menezes
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  2. S Z Qasim
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Menezes, M.R., Qasim, S.Z. Use of electrophoresis and immunoelectrophoresis in taxonomic and pollution studies. Proc Ani Sci 93, 179–198 (1984). https://doi.org/10.1007/BF03186279

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