Advertisement

Chinese Science Bulletin

, Volume 47, Issue 19, pp 1629–1633 | Cite as

Photosynthetic bicarbonate utilization inPorphyra haitanensis (Bangiales, Rhodophyta)

  • Dinghui Zou
  • Kunshan GaoEmail author
Notes

Abstract

The activities of carbonic anhydrase (CA) and photosynthesis ofPorphyra haitanensis were investigated in order to see its photosynthetic utilization of inorganic carbon source. Both intra- and extra-cellular CA activities existed in the thallus. CA inhibitors, acetazolamide (AZ) and ethoxy-zolamide (EZ), remarkably depressed the photosynthetic oxygen evolution in seawater of pH 8.2 and 10.0, and EZ showed stronger inhibition than AZ. The observed net photosynthetic rate in seawater of pH 8.2 was much higher than that of CO2 supply theoretically derived from spontaneous dehydration of HCO3 .P. haitanensis also showed a rather high pH compensation point (9.9). The results demonstrated thatP. haitanensis could utilize bicarbonate as the external inorganic carbon source for photosynthesis. The bicarbonate utilization was closely associated with HCO3 dehydration catalyzed by extracellular CA activity. The inorganic carbon composition in seawater could well saturate the photosynthesis ofP. haitanensis. The low Km value and compensation points for inorganic carbon reflected the existence of CO2-concentrating mechanism in this alga.

Keywords

Porphyra haitanensis photosynthesis bicarbonate inorganic carbon utilization carbonic anhydrase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Raven, J. A., Inorganic carbon acquisition by marine autotrophs, Adv. Bot. Res., 1997, 27: 85.CrossRefGoogle Scholar
  2. 2.
    Falkowski, P. G., Raven, J. A., Aquatic Photosynthesis, Blackwell Science, Malden, MA: Capital City Press, 1997.Google Scholar
  3. 3.
    Hanelt, D., Huppertz, K., Nultsch, W., Daily course of photosynthesis and photoinhibition in marine macroalgae investigated in the laboratory and field, Mar. Ecol. Prog. Ser., 1993, 97: 31.CrossRefGoogle Scholar
  4. 4.
    Gao, K., McKinley, K. R., Use of macroalgae for marine biomass production and CO2 remediation: A review, J. Appl. Phycol., 1994, 6: 45.CrossRefGoogle Scholar
  5. 5.
    Larsson, C., Axelsson, L., Bicarbonate uptake and utilization in marine macroalgae, Eur. J. Phycol., 1999, 34: 79.CrossRefGoogle Scholar
  6. 6.
    Haglund, K., Bjork, M., Ramazanov, Z. et al., Role of external carbonic anhydrase in light-dependent alkalization byFucus serratus L. andLaminaria saccharina (L.) Lamour, (Phaeophyta), Planta, 1992, 188: 1.CrossRefGoogle Scholar
  7. 7.
    Yue, G. F., Wang, J. X., Wang, J. F. et al., Inorganic carbon acquisition by juvenile sporophyte of Laminarials (L. Japonica ×L. Longissima), Oceanol Limnol Sinica (in Chinese with English summary), 2001, 32(6): 647.Google Scholar
  8. 8.
    Zou, D., Gao, K., Progress in studies on photosynthetic inorganic carbon utilization in marine macroalgae, Marine Science Bulletin (in Chinese with English summary), 2001, 20(5): 83.Google Scholar
  9. 9.
    Axelsson, L., Ryberg, H., Beer, S., Two modes of bicarbonate utilization in the marine green macroalgaUlva lacturca, Plant Cell Environ., 1995, 18: 439.CrossRefGoogle Scholar
  10. 10.
    Larsson, C., Axelsson, L., Ryberg, H. et al., Photosynthetic carbon utilization byEnteromorpha intestinalis (Chorophyta) form a Swedish rockpool, Eur. J. Phycol., 1997, 32: 49.CrossRefGoogle Scholar
  11. 11.
    Axelsson, L., Larsson, C., Ryberg, H., Affinity, capacity and oxygen sensitivity of the two different mechanisms for bicarbonate utilization inUlva lactuca L. (Chlorophyta), Plant Cell Environ., 1999, 22: 969.CrossRefGoogle Scholar
  12. 12.
    Johnston, A. M., Maberly, S. C., Raven, J. A., The acquisition of inorganic carbon by four red macroalgae, Oecologia, 1992, 92: 317.CrossRefGoogle Scholar
  13. 13.
    Giordano, M., Maberly, S. C., Distribution of carbonic anhydrase in British marine macroalgae, Oecologia, 1989, 81: 534.CrossRefGoogle Scholar
  14. 14.
    Gao, K., Research techniques and methods in characterizing photosynthetic carbon fixation by algae, Marine Science (in Chinese), 1999(6): 37.Google Scholar
  15. 15.
    Axelsson, L., Mercado, J., Figueroa, F., Utilization of HCO3 at high pH by the brown macroalgaLaminaria saccharina, Eur. J. Phycol., 2000, 35: 53.Google Scholar
  16. 16.
    Moroney, J. V., Bartlett, S. G., Samuelsson, G., Carbonic anhydrases in plants and algae, Plant Cell Environ., 2001, 24: 141.CrossRefGoogle Scholar
  17. 17.
    Stumm, W., Morgan, J. J., Aquatic Chemistry, 3rd ed., New York: John Wiley and Sons, 1996.Google Scholar
  18. 18.
    Johnson, K. S., Carbon dioxide hydration and dehydration kinetics in seawater, Limol. Oceanogr., 1982, 27: 849.CrossRefGoogle Scholar
  19. 19.
    Axelsson, L., Uusitalo, J., Carbon acquisition strategies for marine macroalgae, Part I: Utilization of proton exchanges visualized during photosynthesis in a closed system, Mar. Biol., 1988, 97: 295.CrossRefGoogle Scholar
  20. 20.
    Maberly, S. C., Raven, J. A., Johnston, A. M., Discriminiation between12C and13C by marine plants, Oecologia, 1992, 91: 481.CrossRefGoogle Scholar
  21. 21.
    Mercado, J. M., Figuroa, F. L., Niell, F. X. et al., A new method for estimating external carbonic anhydrase activity in marcoalgae, J. Phycol., 1997, 33: 999.CrossRefGoogle Scholar
  22. 22.
    Kaplan, A., Reinhold, A., CO2 concentrating mechanism in photosynthetic microorganism, Annu. Rev. Plant Physiol. Plant Mol. Biol., 1999, 50: 539.CrossRefGoogle Scholar
  23. 23.
    Maberly, S. C., Exogenous sources of inorganic carbon for photosynthesis by marine macroalgae, J. Phycol., 1990, 26: 439.CrossRefGoogle Scholar
  24. 24.
    Cook, C. M., Lanaras, T., Colman, B., Evidence for bicarbonate transport in species of red and brown macrophytic marine algae, J. Exp. Bot., 1986, 37: 977.CrossRefGoogle Scholar
  25. 25.
    Maberly, S. C., Carbonate ions appear to neither inhibit nor stimulate use of bicarbonate ions in photosynthesis byUlva lactuca, Plant Cell Environ., 1992, 15: 255.CrossRefGoogle Scholar
  26. 26.
    Kerby, N. W., Raven, J. A., Transport and fixation of inorganic carbon by marine algae, Adv. Bot. Res., 1985, 11: 71.CrossRefGoogle Scholar
  27. 27.
    Johnston, A. M., Raven, J. A., The utilization of bicarbonate ions by the macroalgaeAscophyllum nodosum (L.) Le Jolis, Plant Cell Environ., 1986, 9: 175.Google Scholar
  28. 28.
    Colman, B., The effect of temperature and oxygen on the CO2 compensation point of the marine algaUlva lactuca, Plant Cell Environ., 1984, 7: 619.Google Scholar
  29. 29.
    Smith, R. G., Bidwell, R. G. S., Mechanism of photosynthetic carbon dioxide uptake by the red macroalgaChondrus crispus, Plant Physiol., 1989, 89: 93.CrossRefGoogle Scholar

Copyright information

© Science in China Press 2002

Authors and Affiliations

  1. 1.Marine Biology Institute, Science CenterShantou UniversityShantouChina
  2. 2.Institute of HydrobiologyChinese Academy of SciencesWuhanChina

Personalised recommendations