Morphological and molecular characterization of twoG. somalense monosomic alien addition lines (MAALs)

Abstract

TwoG. somalense monosomic alien addition lines were identified from the derived backcross progenies of allohexaploid betweenG. hirsutum andG. somalense through cytological and morphological observation. Furthermore, the alien addition chromosome was identified and distinguished by RAPD analysis. A total of 160 RAPD primers were used for PCR amplification. Primer SBSG11 could produce a specific molecular marker (600 bp) for monosomic alien addition line I (MAAL I). Primer SBSC03 could produce a specific molecular marker (700 bp) for monosomic alien addition line II(MAAL II). SBSE07 and SBSE08 could respectively produce common molecular marker for monosomic alien addition lines I and II.G. somalense alien addition lines could be important for cotton improvement.

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References

  1. 1.

    Knight, R. L., D. Sc (Lond), The distribution of wild species ofGossypium in the Sudan, Cotton Grow Rev., 1949, 26: 278–285.

    Google Scholar 

  2. 2.

    Saunders, J. H., The wild species ofGossypium II, the C and E genomes, Cotton Grow Rev., 1959, 36: 166–178.

    Google Scholar 

  3. 3.

    Douwes, H., The cytological relationships ofGossypium areysianum Deflens, Journal of Genetics, 1953, 51: 611–624.

    Article  Google Scholar 

  4. 4.

    Phillips, L. L., The cytology and phylogenetics of the diploid species ofGossypium, Amer. J. Bot., 1966, 53(4): 328–335.

    Article  Google Scholar 

  5. 5.

    Xiang, S. K., Yang, W. H., Studies on gossypol and its enantiomers in the seeds of cottonGossypium, Scientia Agricultural Sinica (in Chinese), 1993, 26(6): 31–35.

    Google Scholar 

  6. 6.

    He Jianxing, Jiang Ruqin, Zhang Xinxue, The cytogenetics and fiber ptoperties of an allohexaploid betweenG. hirsutum andG. somalense, Chinese Science Bulletin, 2000, 45(16): 1742–1747.

    Google Scholar 

  7. 7.

    Rooney, W. L., Stelly, D. M., Identification of fourGossypium sturianum monosomic alien addition derivatives from a backcrossing program withG. hirsutum, Crop Sci., 1991, 31: 337–341.

    Google Scholar 

  8. 8.

    Williams, J. G., Kubelik, A. R., Livak, K. J. et al., DNA polymorphisms amplified by arbitrary primers are useful as genetic markers, Nucleic Acids Res., 1990, 18: 6531–6535.

    Article  Google Scholar 

  9. 9.

    Welsh, J., McClelland, M., Fingerprinting genomes using PCR with arbitrary primers, Nucleic Acids Res., 1990, 18: 7213–7218.

    Article  Google Scholar 

  10. 10.

    Liang Zhenglan, Jiang Ruqin, Zhao Guozhong et al., Studies on the trispecific hybrid (Gossypium barbadense-G. thurberi-G. hirsutum) and new variety breeding, Acta agronomica Sinica (in Chinese), 1996, 22(6): 673–680.

    Google Scholar 

  11. 11.

    Paterson, A. H. Brubaker, L., Wendel, J. et al., A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis, Plant Molecular Biology Report, 1993, 11(2): 122–127.

    Article  Google Scholar 

  12. 12.

    Altman, D. W., Stelly, D. M., Kohel, R. J., Introgression of the glanded-plant and glandless-seed trait fromGossypium sturtianum Willis into cultivated upland cotton using ovule culture, Crop Sci., 1987, 27: 880–884.

    Article  Google Scholar 

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Correspondence to Jianxing He.

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These two authors contributed equally to this work.

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Zhou, Z., Yu, P., Liu, G. et al. Morphological and molecular characterization of twoG. somalense monosomic alien addition lines (MAALs). Chin. Sci. Bull. 49, 910–914 (2004). https://doi.org/10.1007/BF03184010

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Keywords

  • G. hirsutum
  • G. somalense
  • MALLs
  • morphology
  • cytology
  • RAPD