Abstract
Somatic cell clone technology is a viable approach to preserving endangered livestock and wildlife genetic resources. In the present research, somatic cell nuclear transfer (SCNT) was performed using granulose cells from the critical endangered Chinese red-cross yellow cattle as donor cells. A total of 211 oocytes were manipulated and 166 (79%) of them were successfully enucleated. 112 (67.4%) SCNT embryos were reconstructed, 94 (83%) of them cleaved, and 48 (43%) of them developed to blastocyst stage. SCNT blastocysts were transferred to 6 Holstein recipients, and 2 (33%) of them were found to be pregnant. One of them maintained to term and delivered a calf, whereas another aborted. Effect of different fusion buffer (mannitol vs. Zimmerman fusion buffer) and different activation methods (calcium ionophore+6-DMAP vs. cycloheximide+CB) on fusion rate and development of SCNT embryos were investigated. The results indicated that: (i) on condition of two DC pulses of 2.5 kV/cm for 10 μs each, fusion rates were higher in mannitol solution than in Zimmerman fusion buffer (71% vs. 61%, respectively, p<0.05=, but the blastocysts rates did not differ between two treatments (36% vs. 39%, p>0.05); (ii) There was no significant difference in development rates to the blastocyst stage for SCNT embryos activated by calcium ionophore+6-DMAP or by cycloheximide+CB (42% vs. 46%, respectively, p>0.05). Microsatellite DNA analysis examining 28 loci confirmed that the cloned calf was genetically identical to the donor Jinan red-cross yellow cattle and different from the recipient females. Growth and reproductive performance of cloned cow were evaluated, and there were no differencei cross-red n it between cloned and normal control Jinan yellow cattle. Furthermore, the cloned yellow cow has delivered a healthy yellow calf.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Schnieke, A. E., Kind, A. J., Ritchie, W. A. et al., Human factor IX transgenic sheep produced by transfer of nuxlei from transfected fetal fibroblast, Science, 1997, 278: 2130–2133.
McCreath, K. J., Howcroft, J., Campbell, K. H. S. et al., Production of gene-targeted sheep by nuclear transfer from cultured somatic cells, Nature, 2000, 405: 1066–1069.
Cibelli, J. B., Stice, S. L., Golueke, P. J. et al., Cloned transgenic calves produced form nonquiescent fetal fibroblasts, Science, 1998, 280: 1256–1258.
Keefer, C. L., Baldassare, H., Keyson, R. et al., Generation of dwarf goat (Capra hircus) clones following nuclear transfer with transfected and nontransfected fetal fibroblasts andin vitro matured oocytes, Biol. Reprod., 2001, 64: 849–856.
Surani, M. A., Reprogramming a somatic nucleus by trans-modification activity in germ cells, Semin. Cell Dev. Biol., 1999, 10: 273–277.
Kuroiwa, Y., Kasinathan, P., Choi, Y. J. et al., Cloned transchromosomic calves producing human immunoglobulin, Nat. Biotechnol., 2002, 20: 889–894.
Shiga, K., Fujita, T., Hirose, K. et al., Production of calves by transfer of nuclei from cultured somatic cells obtained from Japanese black bulls, Theriogenology, 1999, 52: 527–535.
Kubota, C., Yamakuchi, H., Todoroki, J. et al., Six cloned calves produced from adult fibroblast cells after long term culture, Proc. Natl. Acad. Sci. USA, 2000, 97: 990–995.
Han, Y. M., Kang, Y. K., Koo, D. B. et al., Nuclear reprogramming of cloned embryos producedin vitro, Theriogenology, 2003, 59(1): 33–44.
Well, D. N., Misicam, P. M., Adult somatic cell nuclear transfer is used to preserve the last surviving cow of the Enderby Island cattle breed, Reprod. Fertil. Dev., 1998, 10: 369–378.
Chen, D. Y., Sun, Q. Y., Liu, J. L. et al., The giant panda (Ailuropoda melanoleuca) somatic cell nucleus can dedifferentiate in rabbit ooplasm and support early development of the reconstructed egg, Science in China, Ser. C., 1999, 42(4): 346–353
Vogel, G., Cloned gaurus with a short-lived success, Science, 2001, 291: 409.
Chen, Y. C., Cao, H. H., Biodiversity and conservation of Chinese yellow cattle breed, Biological Diversity (in Chinese), 2001, 9(3): 275–283.
Ma, Y. H., Chen, Y. C., Feng, W. Q., A general introduction of biodiversity of Chinese livestock, Acta Veterinaria et Zootechnica Sinica (in Chinese), 2000, 31(5): 397–398.
Gong, G., Dai, Y., Fan, B. et al., Production of transgenic blastocysts by nuclear transfer from different types of somatic cells in cattle, Science in China, Ser. C., 2004, 47(2): 183–189.
Gong, G., Dai, Y., Fan, B. et al., Production of transgenic claves by somatic cell nuclear transfer, Chinese Science Bulletin, 2004, 49(2): 161–166.
Gong, G., Dai, Y., Zhu, H. et al., Generation of cloned calves from different types of somatic cells, Science in China, Ser. C., 2004, 47(5): 470–476.
Zimmermann, U., Vienken, J., Electric field-induced cell-to-cell fusion, J. Membr. Biol., 1982, 67(3): 165–182.
Gong, G., Dai, Y., Fan, B. et al., Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer, Mol. Reprod. Dev., 2004, 69(3): 278–288.
Heaton, M. P., Harhay, G. P., Bennett, G. L. et al., Slection and use of SNP markers for animal identification and paternity analysis in U.S. beef cattle, Mammalian Genome, 2002, 13: 272–281.
Holt, C. L., Stauffer, C., Wallin, J. M. et al., Practical applications of genotypic surveys for forensic STR testing, Forensic Sci. Int., 2000, 112: 91–109.
Ji, Y. L., ed., Applied Cattle Breeding (in Chinese), Beijing: China Agriculture Press, 2001.
Zheng, P. L., Chinese Cattle Breed (in Chinese), Beijing: China Agriculture Press, 2001
Lanza, R. P., Cibelli, J. B., Diaz, F. et al., Cloning of an endangered species, Cloning, 2000, 2: 79–90.
Heyman, Y., Chavatte-Palmer, P., LeBourhis, D. et al., Frequency and occurrence of late-gestation losses from cattle cloned embryos, Biol. Reprod., 2002, 66, 6–13.
Hill, J. R., Burghard, R. C., Jones, K. et al., Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses, Biol. Teprod., 2000, 63, 1787–1794.
Wilson, J. M., Williams, J. D., Bondioli, K. R. et al., Comparison of birth weight and growth characteristics of bovine calves produced by nuclear transfer (cloning), embryo transfer and natural mating, Anim. Reprod. Sci., 1995, 38: 73–83.
Garry, F. B., Adams, R., McCann, J. P., Odde KG, Postnatal characteristic of claves produced by nuclear transfer cloning, Theriogenlolgy, 1996, 45: 141–152.
Kang, Y. K., Koo, K. B., Park, J. S. et al., Aberrant methylation of donor genome in cloned bovine embryos, Nat. Genet., 2001, 28: 173–177.
Kato, Y., Tani, T., Tsunoda, Y. et al., Aberrant patterns of X chromosome inactiviation in bovine clones, Nat. Genet., 2002, 31: 216–219.
Wrenzycki, C., Wells, D., Herrmann, D. et al., Nuclear transfer protocol affects messenger RNA expression patterns in cloned bovine blastocysts, Biol. Reprod., 2001, 65: 309–317.
Author information
Authors and Affiliations
Corresponding author
About this article
Cite this article
Dai, Y., Lian, Z., Zhu, H. et al. Clone of Chinese Jinan redcross yellow cattle and evaluation of reproductive characteristics of cloned calf. Chin. Sci. Bull. 50, 2592–2597 (2005). https://doi.org/10.1007/BF03183656
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF03183656