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The equal-efficiency-proving of fluorescence quenching and enhancement equation

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Chinese Science Bulletin

Abstract

Through the fluorescence and ultravioletvisible spectrophotometry we have studied the action of cefoperazone sodium and worfloxacin, two kinds of medicine with albumin, and examined the equal efficiency quality of fluorescence enhancement and fluorescence quenching equation. The result shows the equal efficiency of calculating dissociation constant. In this article, we obtain the dissociation constants of the action of the two kinds of medicine with bovine serum albumin, energy-transfer efficiency, the distance of donor-acceptor and quenching constants. The above shows that the action is not caused by dynamic collision but by the binding of medicine and albumin. This article specially points out that the [Mt]/[Pt], which is the ratio of the maximum mol concentration of the quenching agent (medicine) to fluorescent (albumin) mol concentration when the quenching ends, is very important because it is the maximum binding number of quenching agent molecule to a fluorescent molecule. There is no more quenching after adding more quenching agent beyond this quantity to fluorescent solution. This quantity is determined directly and strictly by experiment, so is also of important significance when discussing quenching efficiency and binding situation of donor-acceptor.

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, 030006, Taiyuan, China

    Manman Yang, Xiaoli Xi & Pin Yang

Authors
  1. Manman Yang
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  2. Xiaoli Xi
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  3. Pin Yang
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Corresponding author

Correspondence to Pin Yang.

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Yang, M., Xi, X. & Yang, P. The equal-efficiency-proving of fluorescence quenching and enhancement equation. Chin. Sci. Bull. 50, 2571–2574 (2005). https://doi.org/10.1007/BF03183652

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  • DOI: https://doi.org/10.1007/BF03183652

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