Skip to main content
SpringerLink
Log in

Changes of intracellular Na+ concentration in erythrocytes caused by pulsed electrical field

  • Published:
Science in China Series C: Life Sciences Aims and scope Submit manuscript

Abstract

Changes of sodium ionic concentration of human erythrocytes applied to pulsed electrical field (PEF) were studied by using shift reagent and NMR spectroscopy. The results show that the concentration of intraceUular Na+ increases with the increasing intensity of PEF when the erythrocytes are applied to PEF with higher intensities. The relationship between intracellular Na+ concentrations and the intensities of PEF does not follow linear or exponential behavior. As the intensities increase, the intracellular Na+ concentrations increase even faster by an exponential curve. However under effects of PEF at lower intensities, intracellular Na+ concentration decreases. Ouabain can inhibit the decrease of intracellular Na+ concentration, and the inhibition increases with the increasing concentration of ouabain, suggesting that Na+, K+-ATPase on cell membrane can be activated by PEF at lower intensities. Direct measurement of activities of the enzyme by using Malachite green method has confirmed this observation. Cell permeabilities to ions, activation of enzymes by electrical fields and transmission of physical signals like PEF across cell membranes are discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kinosita, K. Jr., Tsong, T. Y., Hemolysis of human erythrocytes by a transient electric field,Proc. Natl. Acad. Sci. USA, 1977, 74(5): 1923.

    Article  CAS  PubMed  Google Scholar 

  2. Riemann, E., Zimmermann, U., Pilwat, G., Release and uptake of haemoglobin and ions in red blood cells induced by dielectric breakdown,Biochem. Biophys. Acta, 1975, 394(3): 449.

    Article  CAS  PubMed  Google Scholar 

  3. Serpersu, E. H., Kinosita, K., Tsong, T. Y., Reversible and irreversible modification of erythrocyte membrane permeability by electric field,Biochem. Biophys. Acta, 1985, 812: 779.

    Article  CAS  PubMed  Google Scholar 

  4. Graziana, A., Ranjeva, R., Teissie, J., External electric fields stimulate the electrogenic calcium/sodium exchange in plant protoplasts,Biochemistry, 1990. 29(36): 8313.

    Article  CAS  PubMed  Google Scholar 

  5. Schwister, K., Deuticke, B., Formation and properties of aqueous leaks induced in human erythrocytes by electrical breakdown,Biochem. Biophys. Acta, 1985, 816: 332.

    Article  CAS  PubMed  Google Scholar 

  6. Schwister, K., Chang, W., Electric-field-induced volume and membrane ionic permeability changes of red blood cells,IEEE Transon Biomedical Engineering, 1993. 40(10): 1054.

    Article  Google Scholar 

  7. Pan Suzhen, Bin Jinfeng, Chen Yunzhun, NMR in combination with an ionic paramagnetic shift reagent (Sr) has been used to study intracellular and extracellular cation concentrations,Acta Biophys. Sinica (in Chinese), 1990, 6(4): 476.

    Google Scholar 

  8. Kong Kejian, Liu Ying, Chang Jinju, Studies on dynamic of electropermeabilization of Tb3 + loaded in human erythrocyte ghosts,Acta Biophysics Sinica (in Chinese), 1994, 10(1): 42.

    CAS  Google Scholar 

  9. Xu Youhan, Song Hua, A simple sensitive assay of the activity of ATPase,Progress in Biochemistry and Biophysics (in Chinese), 1986, 13(4): 64.

    Google Scholar 

  10. Tsong, T.Y., Electrical modulation of membrane proteins,Annu. Rev. Biophys. Chem., 1990,19: 83.

    Article  CAS  Google Scholar 

  11. Lingrel, J.B., Kuntzweiler, T., Na+, K+-ATPase,J. Biol. Chem., 1994,269: 19659.

    CAS  PubMed  Google Scholar 

  12. Blank, M., Electrical effects of environmental electromagnetic fields: Molecular mechanisms,Biosystems, 1995,35: 2.

    Article  Google Scholar 

  13. Derijard, B., Hibi, M., Wu, I.et al., JINK1: A protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain,Cell, 1994,76: 1025.

    Article  CAS  PubMed  Google Scholar 

  14. Kyriakis, J.M., Banerjee, P., Nikolakaki, T.et al., The stress-activated protein kinase subfamily of c-Jun kinases,Nalure, 1994,369: 156.

    CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Chinese Academy of Sciences, Institute of Biophysics, 100101, Beijing, China

    Jinzhu Zhang, Tong Sun, Ya Chen, Shuhua Zhou, Yunjun Chen & Suzhen Pang

Authors
  1. Jinzhu Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tong Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ya Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Shuhua Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yunjun Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Suzhen Pang
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Project supported by the National Natural Science Foundation of China and the Analytical and Test Center, Beijing.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Zhang, J., Sun, T., Chen, Y. et al. Changes of intracellular Na+ concentration in erythrocytes caused by pulsed electrical field. Sci. China Ser. C.-Life Sci. 40, 488–495 (1997). https://doi.org/10.1007/BF03183587

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF03183587

Keywords

Search

Navigation