Abstract
Based on the fact that the resonant frequency of a piezoelectric crystal is the function of its surface deposit, and that the primer extends after it hybridizes with the template, the primer extension gene sensor technique was developed. The prominent feature of the technique is that fast and sensitive frequency signals are used as the monitoring system of gene hybridization and primer strand extension. Results show that this technique may be used in homologous analysis of nucleic acid, trace DNA detection, and determining the integration of DNA. It may also be used for isolation of target gene, gene mutation analysis, and predicting the location of a gene in its genome.
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References
Graham, C.R., Leslie, D., Squirrell, D.J., Gene probe assays on a fibrfoptic evanescent wave biosensor,Biavnsorsand Bioelectronics. 1992, 7(7): 487.
Millan, K.M., Mikkelsen, S. R., Sequenc—selective biosensor for DNA based on electtoactive hybridization indicators,Analytical Uumistry, 1993, 65(17): 2317.
Millan, K.M., Saraullo, A., Mikkelsen, S.R., Voltammetric DNA biosensor for cystic fibrosis based on a modified carbon paste electrode.Analytical Chemistry, 1994. 66(18): 2943.
Guilbault, G.G., Determination of formaldehyde with an enzyme-coated piezoelectric crystal detector,Analytical Chemistry, 1983, 55: 1682.
Chen, Y. H., Hong, W. L., Chen, Q. X.et al., Preparation and application of specific enzyme-digested fragment probe of HSV DNA.Chinese J. Public Haalth (in Chinese). 1991. 10(4): 248.
Hiroshi Sskaoka, Ryo Honda, Takashi Kawana, Detection and typing of herpes simplex virus genomes using polymerase chain reaction,Clinical Virology (in Japanese), 1990, 18(13): 326.
Chen, Y.H., Song, J.D., A simple and practical method of preparation of RNA. inMedical Biologic Research in China (in Chinese), Chengdu: Sichuan Scientific and Technologic Press, 1995, 235.
Maki, R.G., Eddy, R.L. Jr., Byers, M.et al., Mapping of the genes for human endoplasmic reticulum heat shock protein gp96/grp94.Somatic Cell and Molecular Genetics, 1993, 19(1): 73.
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Project supported by the National Natural Science Foundation of China
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Chen, Y., Song, J. & Li, D. Study on gene sensor based on primer extension. Sci. China Ser. C.-Life Sci. 40, 463–469 (1997). https://doi.org/10.1007/BF03183583
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DOI: https://doi.org/10.1007/BF03183583