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Configuration of nucleolar DNAin situ in nucleolus ofAllium cepa cells

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Chinese Science Bulletin

Abstract

The location and configuration of nucleolar DNA have not been determined for a long time. In this paper, we have observed the nucleolar ultrastructure and the character of nucleolar DNA inAllium cepa cells by conventional electron microscopy and the cytochemical NAMA-Ur DNA specific staining method. Furthermore, we have properly improved the NAMA-Ur method so as to analyze the location and configuration of nucleolar DNAin situ. Our results indicated that the nucleolar DNA inAllium cepa cells is mainly located at the border between fibrillar centers and dense fibrillar component, especially distributed in the configuration of encircling the fibrillar centers.

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Correspondence to Shui Hao.

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Tao, W., He, J., Jiao, M. et al. Configuration of nucleolar DNAin situ in nucleolus ofAllium cepa cells. Chin.Sci.Bull. 46, 62–66 (2001). https://doi.org/10.1007/BF03183211

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  • DOI: https://doi.org/10.1007/BF03183211

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