Abstract
It was found that the supplement of 10−4 mol/L AgNO3, in N6 medium enhanced the induction of type I embryogenic calli from immature embryos of maize inbred P9-10. After high-osmotic treatment, the induced calli was taken as transformation recipient to be bombarded with plasmid pMG6 carrying synthetic Bt gene by PDS-1000/He genegun. A total of 14 resistant calli were obtained after screening subsequently on the mediums with gradually increasing selective pressure. 10 plants were regenerated from the resistant calli on 3 different induction media, and 8 out of the 10 regenerated plants were confirmed to be integrated with Bt gene by PCR and Southern blotting analysis. Results of ELSA showed that the Bt-protein content in the leaves of the transgenic plants varied between 20–200 ng/g fresh weight.
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Zhou, F., Wang, G., Xie, Y. et al. Establishment of a genetic transformation system for maize inbred P9-10. Chin. Sci. Bull. 44, 624–627 (1999). https://doi.org/10.1007/BF03182723
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DOI: https://doi.org/10.1007/BF03182723