Abstract
The expression of xylanases and cellulases is under carbon catabolite repression, a regulatory repression mechanism of transcription of these enzymes caused bycre1 gene. In the present study we isolatedcre1 partial gene from a thermophilic fungusChaetomium thermophilum ATCC 28076. The fungus was grown on Eggins and Pugh medium with glucose as a carbon source. Genomic DNA was isolated by two different methods and the integral DNA was subjected to polymerase chain reaction (PCR) for the amplification ofcre1 partial gene sequence. The PCR product was ligated into pTZ57R/T vector and transformed in E.coli TOP 10 strain. cDNA from total RNA was utilized as template for RT-PCR analysis, which confirmed the presence of an intronless cre1 gene in the model organism. This is the first report on isolation ofcre1 gene fragment in C.thermophilum.
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Mushtaq, Z., Saadia, M., Anjum, R.S. et al. Cloning of an intronlesscre1 gene fromChaetomium thermophilum . Ann. Microbiol. 59, 785–788 (2009). https://doi.org/10.1007/BF03179224
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DOI: https://doi.org/10.1007/BF03179224