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Isolation and characterization of arsenic resistant bacteria from tannery wastes and agricultural soils in Thailand

  • Ecological and Environmental Microbiology
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Abstract

Highly arsenic resistant bacteria (27 isolates), which had a minimum inhibitory concentrations (MICs) for arsenite and arsenate of ⩾ 40 mM and > 400 mM, respectively, were isolated from tannery wastes and agricultural soils collected in Central Thailand. On the basis of the morphological, cultural, physiological and biochemical characteristics, and on the principal ubiquinone component and 16S rRNA gene sequence analyses, they were identified as nine isolates each ofKlebsiella (Groups 1 and 8) andAcinetobacter (Groups 2, 3 and 7), four isolates each ofPseudomonas (Groups 4 and 6) andComamonas (Group 5), and one isolate ofEnterobacter (Group 9). From these 27 isolates, only one isolate, A3-3 from the genusComamonas, appeared potentially capable of oxidizing arsenite to arsenate, as determined by silver nitrate staining of arsenite agar plates after colony growth.

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Authors and Affiliations

  1. Inter-department of Environmental Science (IES), Graduate School, 10330, Bangkok, Thailand

    Kitja Chitpirom

  2. Department of Microbiology, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand

    Kitja Chitpirom & Ancharida Akaracharanya

  3. Faculty of Pharmaceutical Sciences, Chulalongkorn University, 10330, Bangkok, Thailand

    Somboon Tanasupawat

  4. Department of Chemistry, Faculty of Science, Chulalongkorn University, 10330, Bangkok, Thailand

    Natchanun Leepipatpibooim

  5. Department of Environmental Science and Engineering, Gwangju Institute of Science and Technology, 500-712, Gwangju, Korea

    Kyoung-Woong Kim

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  1. Kitja Chitpirom
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  2. Ancharida Akaracharanya
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Correspondence to Ancharida Akaracharanya.

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Chitpirom, K., Akaracharanya, A., Tanasupawat, S. et al. Isolation and characterization of arsenic resistant bacteria from tannery wastes and agricultural soils in Thailand. Ann. Microbiol. 59, 649–656 (2009). https://doi.org/10.1007/BF03179204

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  • DOI: https://doi.org/10.1007/BF03179204

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