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Pancreatic duct secretion: experimental methods, ion transport mechanisms and regulation

Secreción pancreática ductular: Métodos experimentales, mecanismos de transporte iónico y regulación (minirrevisión)

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Abstract

The pancreatic ductal tree conveys enzymatic acinar products to the duodenum and secretes the fluid and ionic components of pancreatic juice. The physiology of pancreatic duct cells has been widely studied, but many questions are still unanswered concerning their mechanisms of ionic transport. Differences in the transport mechanisms operating in the ductal epithelium has been described both among different species and in the different regions of the ductal tree. In this review we summarize the methods developed to study pancreatic duct secretion both in vivo and in vitro, the different mechanisms of ionic transport that have been reported to date in the basolateral and luminal membranes of pancreatic ductal cells and the regulation of pancreatic duct secretion by nervous endocrine and paracrine influences.

Resumen

El árbol ductal pancreático transporta las enzimas pancreáticas de origen acinar hasta el duodeno y secreta el componente hidroelectrolítico del jugo pancreático. Aunque existen numerosos estudios sobre la fisiología de las células ductulares pancreáticas todavía quedan muchas cuestiones sin resolver con respecto a los mecanismos de transporte iónico en estas células. Existen diferencias en los mecanismos de transporte iónico en el epitelio ductular, tanto entre las diferentes especies estudiadas como entre las distintas zonas del árbol ductal. En esta revisión se hace un resumen de los diferentes métodos aplicados al estudio de la secreción pancreática ductular, tanto in vivo como in vitro; se describen los distintos mecanismos de transporte iónico, tanto en la membrana basolateral como en la luminal, descritos hasta la fecha en las células ductulares; y, finalmente, se detallan los mecanismos de regulación, nerviosos, endocrinos y paracrinos de la secreción pancreática ductular.

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  1. Departamento de Fisiología y Farmacología, Universidad de Salamanca, Salamanca, Spain

    M. García, P. Hernández-Lorenzo, J. I. San Román & J. J. Calvo

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  1. M. García
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  2. P. Hernández-Lorenzo
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García, M., Hernández-Lorenzo, P., San Román, J.I. et al. Pancreatic duct secretion: experimental methods, ion transport mechanisms and regulation. J Physiol Biochem 64, 243–257 (2008). https://doi.org/10.1007/BF03178846

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