Abstract
To find a high γ-aminobutyric acid-producing lactic acid bacterium, more than 1000 strains of lactic acid bacteria isolated frompaocai samples in various areas of China were screened by the ability in production of γ-aminobutyric acid, analysed with paper chromatography, HPLC and HPLC-MS. Among them, one strain NCL912 exhibited high ability to convert sodium glutamate to γ-aminobutyric acid. The strain accumulated 149.05 mM of γ-aminobutyric acid in a modified MRS medium containing 3% sodium glutamate after 48 h of static cultivation at 30 °C. This strain was identified asLactobacillus brevis according to its phenotypic and phylogenetic characteristics.
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Adeghate E., Ponery A.S. (2002). GABA in the endocrine pancreas: cellular localization and function in normal and diabetic rats. Tissue Cell, 34: 1–6.
Hagiwara H., Seki T., Ariga T. (2004). The effect of pre-germinated brown rice intake on blood glucose and PAI-1 levels in streptozotocin-induced diabetic rats. Biosci. Biotechnol. Biochem., 68: 444–447.
Hao R., Schmit J.C. (1993). Cloning of the gene for glutamate decarboxylase and its expression during conidiation inNeurospora crassa. Biochem. J., 293: 735–738.
Huang J., Mei L., Sheng Q. Yao S., Lin D. (2007a). Purification and characterization of glutamate decarboxylase oflactobacillus brevis CGMCC 1306 isolated from fresh milk. Chinese J. Chem. Eng., 15: 157–161.
Huang J., Mei L., Xia J. (2007b). Application of artificial neural network coupling particle swarm optimization algorithm to biocatalytic production of GABA. Biotechnol. Bioeng., 96: 924–931.
Jakobs C., Jaeken J., Gibson K.M. (1993). Inherited disorders of GABA metabolism. J. Inherit. Metab. Dis., 16: 704–715.
Komatsuzaki N., Shima J., Kawamoto S., Momose H., Kimura T. (2005). Production of γ-aminobutyric acid (GABA) byLactobacillus paracasei isolated from traditional fermented foods. Food Microbiol., 22: 497–504.
Kono I., Himeno K. (2000). Changes in α-aminobutyric acid content during beni-koji making. Biosci. Biotechnol. Biochem., 64: 617–619.
Manyam B.V., Katz L., Hare T.A., Kaniefski K., Tremblay R.D. (1981). Isoniazid-induced elevation of cerebrospinal fluid (CSF) GABA levels and effects on Chorea in Huntington’s disease. Ann. Neurol., 10: 35–37.
Maras B., Sweeney G., Barra D., Bossa F., John R.A. (1992). The amino acid sequence of glutamate decarboxylase fromEscherichia coli. Eur. J. Biochem., 204: 93–98.
Nomura M., Kimoto H., Someya Y., Furukawa S., Suzuki I. (1998). Production of γ-aminobutyric acid by cheese starters during cheese ripening. J. Dairy Sci., 81: 1486–1491.
Nomura M., Nakajima I., Fujita Y., Kobayashi M., Kimoto H., Suzuki I., Aso H. (1999).Lactococcus lactis contains only one glutamate decarboxylase gene. Microbiology, 145: 1375–1380.
Nomura M., Kobayashi M., Ohmomo S., Okamoto T. (2000). Inactivation of the glutamate decarboxylase gene inLactococcus lactis subsp. cremoris. Appl. Environ. Microbiol., 66: 2235–2237.
Park K.B., Oh S.H. (2007). Production of yogurt with enhanced levels of gamma-aminobutyric acid and valuable nutrients using lactic acid bacteria and germinated soybean extract. Biores. Technol., 98: 1675–1679.
Sethi M.L. (1999). Enzyme inhibition XI: glutamate decarboxylase activity relationship with the reaction products as determined by the colorimetric and radioisotopic methods. J. Pharm. Biomed. Anal., 19: 847–54.
Siragusa S., De Angelis M., Di Cagno R., Rizzello C.G., Coda R., Gobbetti M. (2007). Synthesis of gamma-aminobutyric acid by lactic acid bacteria isolated from a variety of Italian cheeses. Appl. Environ. Microbiol., 73: 7283–7290.
Smith D.K., Kassam T., Singh B., Elliott J.F. (1992).Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci. J. Bacteriol., 174: 5820–5826.
Stackebrandt E., Goebel B.M. (1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int. J. Syst. Bacteriol., 44: 846–849.
Tanasupawat S., Shida O., Okada S., Komagata K. (2000).Lactobacillus acidipiscis sp. nov. andWeissella thailandensis sp. nov., isolated from fermented fish in Thailand. Int. J. Syst. Evol. Microbiol., 50: 1479–1485.
Ueno Y., Hayakawa K., Takahashi S., Oda K. (1997). Purification and characterization of glutamate decarboxylase fromLactobacillus brevis IFO 12005. Biosci. Biotech. Biochem., 61: 1168–1171.
Van der Meulen R., Scheirlinck I., Van Schoor A., Huys G., Vancanneyt M., Vandamme P., De Vuyst L. (2007). Population dynamics and metabolite target analysis of lactic acid bacteria during laboratory fermentations of wheat and spelt sourdoughs. Appl. Environ. Microbiol., 73: 4741–4750.
Wong C.G., Bottiglieri T., Snead O.C. 3rd (2003). GABA, γ-hydroxybutyric acid, and neurological disease. Ann. Neurol., 6: 3–12.
Yokoyama S., Hiramatsu J.I., Hayakawa K. (2002). Production of γ-Aminobutyric acid from alcohol distillery lees byLactobacihs brevis IFO-12005. J. Biosci. Bioeng. 93: 95–97.
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Li, H., Gao, D., Cao, Y. et al. A high γ-aminobutyric acid-producingLactobacillus brevis isolated from Chinese traditionalpaocai . Ann. Microbiol. 58, 649–653 (2008). https://doi.org/10.1007/BF03175570
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DOI: https://doi.org/10.1007/BF03175570