Abstract
Fourteen seaweeds collected from the intertidal zone of Southwest coast of India were tested against ten human pathogen bacteria and one human pathogen fungus using the well diffusion test in the casitone agar medium. The species used in the present study include five Chlorophyta (Bryopsis plumosa, Ulva fasciata, Acrosiphonia orientalis, Chaetomorpha antennina, Grateloupia filicina), five Rhodophyta (Hypnea pannosa, Gracilaria corticata, Centroceras clavulatum, Portieria hornemannii, Cheilosporum spectabile) and four Phaeophyta (Padina tetrastromatica, Sargassum wightii, Stocheospermum marginatum, Chnoospora bicanaliculata). Of these, seven species were determined to be highly bioactive and screened on the multiresistant pathogens. We found that drying process has eliminated the active principles in the seaweeds. In the present study, methanol:toluene (3∶1) was found to be the best solvent for extracting the antimicrobial principles from fresh algae. However, the ethanolic extract showed no antibacterial activity.Acrosiphonia orientalis showed activity against 70% of the tested organisms.Stocheospermum marginatum was the only seaweed that showed activity againstKlebsiella pneumoniae. The extract fromGracilaria corticata was highly active againstProteus mirabilis, a Gram negative pathogenic bacterium. The present findings revealed that the tested seaweeds were highly active against Gram negative bacteria than Gram positive bacteria. The antimicrobial principle from seaweed was found to be a lipophilic compound. The compound was stable over a wide range of temperature (30–60 °C). The active principles of highly active seaweedsAcrosiphonia orientalis andStocheospermum marginatum were bactericidal.
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References
Awad N.E. (2000). Biologically active steroid from the green algaeUlva lactica. Phytother. Res., 14: 641–643.
Ballatine D.I., Gerwick W.H., Velez S.M., Alexander E., Guevara P. (1987). Antibiotic activity of lipid soluble extracts from Caribbean marine algae. Hydrobiologia, 151: 463–469.
Campos-Takaki G.M., Diu M.B.S., Koening M.I., Pereira E.C. (1988). Screening of marine algae from Brazilian northeastern coast for antimicrobial activity. Bot. Mar., 31: 375–377.
Egorov N.S. (1957). Determination of antibiotic activity of micro-organisms by the agar block method. Antibioki, 2: 50–53.
Fables C.I., Arias A., Gill-Rodriguez M.C. (1995).In vitro study of antimicrobial activity in algae (Chlorophyta, Phaeophyta, Rhodophyta) collected from the coast of Tenerife (in Spanish). Anuario del Estudios Canneries, 34: 181–192.
Fenical W., Paul V.S. (1984). Antimicrobial and cytotoxic terpenoids from tropical green algae of the family Udoteaceae. In: Bird C.J., Ragan M.A., Eds, 11th International Seaweed Symposium, Dr. W. Junk Publishers, Dordrccht, Boston, Lancaster, pp. 135–140.
Forbes B.A., Sahm D.F., Weissfeld A.S., Trevino E.A. (1990). Methods for testing antimicrobial effectiveness. In: Baron E.J.et al., Eds, Bailey and Scott’ Diagnostic Microbiology, Mosby Co, St Louis, Missouri, pp. 171–194.
Gerwick W.H., Fenical W., Norris J.N. (1985). Chemical variation in the tropical seaweedStypopodium zonale (Dictyotaceae). Phytochemistry, 24: 1279–1283.
Glombitza K.W. (1979). Antibiotics from algae. In: Hoppe I.L.A., Ed., Marine Algae in Pharmaceutical Science, Waiter de Gruyter, Berlin, New York, 303f.
Gonzalez del Val A., Platas G., Basillio A. (2001). Screening of antimicrobial activities in red, green, and brown algae from Gran Canaria (Canary Islands, Spain). Int. Microbiol., 4: 35–40.
Harder R. (1917). Ernahrungphysiologische untersuchunghen an Cyanophyceen, Hauptsachlich am endophytischenNostoc punctiformie. Z. Bot., 9: 145.
Hay M.E. (1996). Marine chemical ecology. What’s known and what’s next? J. Exp. Mar. Biol. Ecol., 200: 103–134.
Konig G.M., Wright A.D., Stiche O., Angerhofer C.K., Pezzuto J.M. (1994). Biological activities of selected marine natural products. Planta Med., 60: 532–537.
Lima-Filho J.V.M., Carvalho A.F.F.U., Freitas S.M. (2002). Antibacterial activity of extracts of six macroalgae from the Northeastern Brazillian coast. Brazilian J. Microbiol., 33: 311–333.
Masuda M., Abe T., Sato S. (1997). Diversity of halogenated secondary metabolites in the red algaeLaurencia nipponica (Rhodomelaceae Ceramiales). J. Phycol., 33: 196–208.
Michanek G. (1979). Seaweed resources for pharmaceutical uses. In: Hoppe I.L.A., Ed., Marine Algae in Pharmaceutical Science, Waiter de Gruyter, Berlin, New York, pp. 203–235.
Olessen P.E., Maretzki A., Almodovar L.A. (1963). An investigation of antimicrobial substances from marine algae. Bot. Mar., 6: 226–232.
Paul V.J., Puglisi M.P. (2004). Chemical mediation of interactions among marine organisms. Nat. Prod. Rep., 21: 189–209.
Perez R.M., Avila J.G., Perez G. (1990). Antimicrobial activity of some American algae. J. Ethnopharmacol., 29: 111–118.
Rosell K.G., Srivastava L.M. (1987). Fattyacids as antimicrobial substances in brown algae. Hydrobiologia, 151: 471–475.
Sastry V.M.V.S., Rao G.R.K. (1994). Antibacterial substances from marine algae: successive extraction using benzene, chloroform and methanol. Bot. Mar., 37: 357–360.
Selvin J. (2002) Shrimp disease management using secondary metabolites isolated from marine organisms. Ph.D dissertation, Manonmaniam Sundaranar University, Tirunelveli, India.
Selvin J., Lipton A.P. (2004). Biopotentials ofUlva fasciata andHypnea musciformis collected from the peninsular coast of India. J. Mar. Sci. Technol., 12: 1–6.
Tuney I, Cadirci BH, Unal D, Sukatar A. (2006). Antimicrobial activities of the extracts of marine algae from the coast of Urla (Imzir, Turkey). Turk. J. Biol., 30: 171–175.
Watson S.B., Cruz-Rivera E. (2003). Algal chemical ecology: an introduction to the special issue. Phycologia, 42: 319–323.
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Shanmughapriya, S., Manilal, A., Sujith, S. et al. Antimicrobial activity of seaweeds extracts against multiresistant pathogens. Ann. Microbiol. 58, 535–541 (2008). https://doi.org/10.1007/BF03175554
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DOI: https://doi.org/10.1007/BF03175554