Abstract
The mutagenic potential of aqueous, Total Oligomers Flavonoids (TOF), ethyl acetate, and methanol extracts as well as essential oil (EO) obtained from tubers ofCyperus rotundus L. was assessed by “Ames assay”, usingSalmonella tester strains TA98 and TA100, and “SOS chromotest” usingEscherichia coli PQ37 strain with and without an exogenous metabolic activation system (S9). None of the different extracts showed a mutagenic effect. Likewise, the antimutagenicity of the same extracts was tested using the “Ames test” and the “SOS chromotest”. Our results showed thatC. rotundus extracts have antimutagenic effects withSalmonella typhimurium TA98 and TA100 strains towards the mutagen Aflatoxin B1 (AFB1), as well as withE. coli PQ37 strain against AFB1 and nifuroxazide mutagens. A free radical scavenging test was used in order to explore the antioxidant capacity of the extracts obtained from the tubers ofC. rotundus. TOF, ethyl acetate and methanol extracts showed an important free radical scavenging activity towards the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. These extracts showed IC50 values of respectively 5, 20 and 65 μg/ml. The beneficial effects of TOF, ethyl acetate, methanol and essential oil extracts ofC. rotundus have been assessed by antioxidant and antimutagenic activities.
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References
Bhattarai N.K. (1993). Folk herbal remedies for diarrhoea and dysentery in central Nepal. Fitoterapia, 64: 243–250.
Bradford M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248–254.
Bruneton J. (1999). Pharmacognosie: Phytochimie, Plantes Médicinales, 3ème éditions, Tec. & Doc. Paris.
Calomme M., Pieters L., Vlietink A., Berghe D.V. (1996). Inhibition of bacterial mutagenesis flavonoids. Planta Med., 62: 222–226.
Cuénod A. (1954). Flore de la Tunisie: Cryptogames Vasculaires, Gymnospermes et Monocotylédones. Office de Expérimentation et de la Vulgarisation Agricoles de Tunisie, Tunis.
De Flora S. (1998). Mechanisms of inhibitors of mutagenesis and carcinogenesis. Mutat. Res., 420: 151–158.
Edenharder R., Grunhage D. (2003). Free radical scavenging abilities of flavonoides as mechanism of protection against mutagenicity induced by test-butyl, hydroperoxide or cumene hydroperoxide inSamonella typhimurium TA102. Mutat. Res., 540: 1–18.
Galati E.M., Miceli N., Taviano M.F., Sanogo R., Raneri E. (2001). Anti-inflammatory and antioxidant activity ofAgeratum conyzoides. Pharm. Biol., 39: 336–339.
Kada T. (1993). Environmental mutagens and cancerogens. In: Sugumura K., Kondo T., Takebe S., Eds., University of Tokyo Press, Tokyo, pp. 355–359.
Kevekordes S., Mersch-Sundermann V., Burghaus C., Spielberger J., Schmeiser H.H., Arlt V.M., Dunkelberg H. (1999). SOS induction of selected naturally occurring substances inEscherichia coli (SOS chromotest). Mutat. Res., 445: 81–91.
Kilani S., Abdelwahed A., Chraief I., Ben Ammar R., Hayder N., Hammami M., Ghedira K., Chekir-Ghedira L. (2005a). Chemical composition, antibacterial and antimutagenic activities of essential oil from (Tunisian)Cyperus rotundus. J. Essent. Oil. Res., 17: 695–700.
Kilani S., Ben Ammar R., Bouhlel I., Abdelwahed A., Hayder N., Ghedira-Chekir L. (2005b). Investigation of extracts from (Tunisian)Cyperus rotundus as antimutagens and radical scavengers. Environ. Toxicol. Pharmacol., 20: 478–484.
Krul C., Luiten-Schuite A., Tenfelde A., van Ommen B., Verhagen H., Havenaar R. (2001). Antimutagenic activity of green tea and black tea extracts studied in a dynamicin vitro gastrointestinal model. Mutat. Res.-Fund. Mol. M., 474: 71–85.
Lee S.E., Shin H.T., Hwang H.J., Kim J.H. (2003). Antioxidant activity of extracts fromAlpinia katsumadai seed. Phytother. Res., 17: 1041–1047.
Lee K.T., Sohn C.I., Park H.J., Kim D.W., Jung G.O., Park K.Y. (2000). Essential moiety for antimutagenic and cytotoxic activity of Hederagenin monodesmosides and bis des mosides isolated from the stem bark ofKalopanax pictus. Planta Med., 66: 329–332.
Maron D.M., Ames B.N. (1983). Revised methods for theSalmonella mutagenicity test. Mutat. Res., 113: 173–215.
Ohira S., Hasegawa T., Hyashi K.I., Hoshino T., Takaoka D., Nozaki H. (1998). Sesquiterpenoids fromCyperus rotundus. Phytochemistry, 47: 1577–1581.
Park K.Y., Jung G.O., Lee K.T., Choi J., Choi M.Y., Kim G.T., Jung H.J., Park H.J.J. (2004). Antimutagenic activity of flavonoids from the heart wood ofRhus verniciflua. Ethnopharmacology, 90: 73–79.
Quillardet P., Huissman O., D’Ari R., Hofnung M. (1982). SOS inEscherichia coli K-12 to measure genotoxicity. Proc. Natl. Acad. Sci USA, 79: 5971–5975.
Quillardet P., Hofnung M. (1985). The SOS chromotest, a colorimetric bacteria assay for genotoxins: procedures. Mutat. Res., 147: 65–78.
Schimmer O., Lindenbaum M. (1992). Tannins with antimutagenic properties in the herb ofAlchemilla species andPotentilla anserina. Planta Med., 47: 466–467.
Shon M.Y., Choi S.D., Kahng G.G., Nam S.H., Sung N.J. (2004). Antimutagenic, antioxidant and free radical scavenging activity of ethyl acetate extracts from white, yellow and red onions. Food Chem. Toxicol., 42: 659–666.
Sonwa M.M., König W.A. (2001). Chemical study of the essential oil ofCyperus rotundus. Phytochemistry, 58: 799–810.
Thebtaranonth C., Thebtaranonth Y., Wanauppathamkul S., Yuthavong Y. (1995). Antimalarial sesquiterpenes from tubers ofCyperus rotundus: structure of 10, 12-peroxy calamenene, a sesquiterpene endoperoxide. Phytochemistry, 40: 125–128.
Tona L., Kambu K., Ngimbi N., Cimanga K., Vlietnick A.J. (1998). Antiamoebic and phytochemical screening of some congolese medical plants. J. Ethnopharmacol., 61: 57–65.
Vaya J., Mohmod S., Goldblum A., Aviram M., Volkavor N., Shaalam A., Musa R., Yagi A., Kabash A., Okamura N., Hrarguchi H., Moustafa S.M., Khalifa T.I. (2002). Antioxidant, free radical scavenging and anti-inflammatory effects of aloesin derivatives in Aloe vera. Planta Med., 68: 957–960.
Yokozozawa T., Chen C.P., Dong E., Tanaka T., Nishioka G.I. (1998). Study on the inhibitory effect of tannins, and flavonoids against DPPH radical. Biochem. Pharmacol., 56: 213–222.
Zani F., Cuzzoni M.T., Doglia M., Benvenuti S., Mazza P. (1993). Inhibition of mutagenicity inSalmonella typhimurium byGlycyrrhiza globra extract, glycyrrhizinic acid, 18α- and 18β-glycyrrhetinic acids. Planta Med., 59: 502–507.
Zeiger E., Risko K.J., Margolin B.H. (1985). Strategies to reduce the cost of mutagenicity screening with theSalmonella assay. Environ. Mutagen., 7: 901–911.
Zhu M., Luk H.H., Fung H.S., Luk C.T. (1997). Cytoprotective effects ofCyperus rotundus against ethanol induced gastric ulceration in rats. Phytother. Res., 11: 392–394, 00000
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Kilani, S., Bouhlel, I., Ben Ammar, R. et al. Chemical investigation of different extracts and essential oil from the tubers of (Tunisian)Cyperus rotundus. Correlation with their antiradical and antimutagenic properties. Ann. Microbiol. 57, 657–664 (2007). https://doi.org/10.1007/BF03175369
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DOI: https://doi.org/10.1007/BF03175369